[Application of second- and third-generation sequencing for bacterial typing].

Abstract

Whole-genome sequencing (WGS) is a modern method that allows deep understanding of studied organisms and is currently gaining importance in molecular microbiology. Data obtained by whole-genome sequencing can be used for a number of different analyses, specifically in bacterial epidemiology. The authors provide an overview of the methods that are used for bacterial typing, description of their principles with subsequent possibilities for evaluation of the obtained data and applications in hospital research.