Some Macrophages With High Expression of CHOP Undergo Necroptosis in Chronic Rhinosinusitis.

IF 2.5 3区 医学 Q1 OTORHINOLARYNGOLOGY American Journal of Rhinology & Allergy Pub Date : 2023-07-01 DOI:10.1177/19458924231163974
Min Li, Ziming Fu, Chenglin Qi, Qinying Wang, Hui Xie, Huabin Li
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引用次数: 1

Abstract

Background: Endoplasmic reticulum stress (ER stress) is activated in chronic sinusitis with nasal polyps (CRSwNP) and leads to increased expression of C/EBP homologous protein (CHOP). However, the role of CHOP in the pathogenesis of CRSwNP remains unclear.

Methods: CHOP expression was detected by immunohistochemistry staining in nasal mucosa of control and CRSwNP patients. Co-localization of CHOP and cleaved caspase3, p-MLKL, and CD68 was detected by immunofluorescence staining in CRSwNP patients. TNFα, IFNγ, IL1β, LPS, and tunicamycin were added to primary dispersed nasal polyp cells (DNPCs) to explore their roles in cell death. Western blot, CCK8 assay, and flow cytometry were employed to detect cell death.

Results: CHOP was specifically activated in CRSwNP compared to controls. It was mainly macrophages that highly expressed CHOP, some of which underwent apoptosis and the other underwent necroptosis. IL1β induced increased CHOP and apoptosis, and a slight p-MLKL. In addition, ER stress could also promote p-MLKL expression. Whereas classical TNFα plus IFNγ and LPS did not induce increased necroptosis in DNPCs.

Conclusion: IL1β induced the apoptotic pathway and minor necroptosis. And ER stress also plays a role in the occurrence of necroptosis in CRSwNP.

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CHOP高表达的巨噬细胞在慢性鼻窦炎中发生坏死性下垂。
背景:内质网应激(ER应激)在慢性鼻窦炎伴鼻息肉(CRSwNP)中被激活,并导致C/EBP同源蛋白(CHOP)的表达增加。然而,CHOP在CRSwNP发病机制中的作用尚不清楚。方法:采用免疫组化染色法检测对照组和CRSwNP患者鼻黏膜CHOP表达。免疫荧光染色检测CRSwNP患者CHOP和cleaved caspase3、p-MLKL和CD68的共定位。将TNFα、IFNγ、il - 1β、LPS和tunicamycin添加到原代分散鼻息肉细胞(DNPCs)中,探讨它们在细胞死亡中的作用。Western blot、CCK8、流式细胞术检测细胞死亡情况。结果:与对照组相比,CHOP在CRSwNP中被特异性激活。高表达CHOP的主要是巨噬细胞,部分细胞发生凋亡,部分细胞发生坏死。il - 1β诱导CHOP和凋亡增加,p-MLKL轻微升高。此外,内质网应激也能促进p-MLKL的表达。而经典的TNFα + IFNγ和LPS不诱导DNPCs的坏死下垂增加。结论:il - 1β可诱导细胞凋亡途径及轻微坏死下垂。内质网应激在CRSwNP坏死性坏死的发生中也起作用。
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来源期刊
CiteScore
5.60
自引率
11.50%
发文量
82
审稿时长
4-8 weeks
期刊介绍: The American Journal of Rhinology & Allergy is a peer-reviewed, scientific publication committed to expanding knowledge and publishing the best clinical and basic research within the fields of Rhinology & Allergy. Its focus is to publish information which contributes to improved quality of care for patients with nasal and sinus disorders. Its primary readership consists of otolaryngologists, allergists, and plastic surgeons. Published material includes peer-reviewed original research, clinical trials, and review articles.
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