Disparity in pseudohyphal morphogenic switching response to the quorum sensing molecule 2-phenylethanol in commercial brewing strains of Saccharomyces cerevisiae.

Scott J Britton, Lisa J Rogers, Jane S White, Hedwig Neven, Dawn L Maskell
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引用次数: 2

Abstract

Saccharomyces cerevisiae can undergo filamentous growth in response to specific environmental stressors, particularly nitrogen-limitation, whereby cells undergo pseudohyphal differentiation, a process where cells transition from a singular ellipsoidal appearance to multicellular filamentous chains from the incomplete scission of the mother-daughter cells. Previously, it was demonstrated that filamentous growth in S. cerevisiae is co-regulated by multiple signaling networks, including the glucose-sensing RAS/cAMP-PKA and SNF pathways, the nutrient-sensing TOR pathway, the filamentous growth MAPK pathway, and the Rim101 pathway, and can be induced by quorum-sensing aromatic alcohols, such as 2-phenylethanol. However, the prevalent research on the yeast-pseudohyphal transition and its induction by aromatic alcohols in S. cerevisiae has been primarily limited to the strain Σ1278b. Due to the prospective influence of quorum sensing on commercial fermentation, the native variation of yeast-to-filamentous phenotypic transition and its induction by 2-phenylethanol in commercial brewing strains was investigated. Image analysis software was exploited to enumerate the magnitude of whole colony filamentation in 16 commercial strains cultured on nitrogen-limiting SLAD medium; some supplemented with exogenous 2-phenylethanol. The results demonstrate that phenotypic switching is a generalized, highly varied response occurring only in select brewing strains. Nevertheless, strains exhibiting switching behavior altered their filamentation response to exogenous concentrations of 2-phenylethanol.

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酿酒酵母商业酿酒菌株假菌丝形态发生切换对群体感应分子2-苯乙醇响应的差异。
酿酒酵母在特定的环境胁迫条件下,特别是氮限制条件下,可以进行丝状生长,因此细胞会进行假菌丝分化,这是一个细胞从单一椭球状外观转变为多细胞丝状链的过程。此前的研究表明,酿酒酵母的丝状生长受多个信号网络的共同调控,包括葡萄糖感应RAS/cAMP-PKA和SNF通路、营养感应TOR通路、丝状生长MAPK通路和Rim101通路,并可由群体感应芳香醇(如2-苯乙醇)诱导。然而,关于酿酒酵母-假菌丝转化和芳香醇诱导的流行研究主要局限于菌株Σ1278b。由于群体感应在商业发酵中的潜在影响,研究了商业酿酒菌株酵母向丝状表型转变的天然变异及其2-苯乙醇的诱导作用。利用图像分析软件对16株在限氮SLAD培养基上培养的商业菌株进行了全菌落成丝的计数;有些添加了外源2-苯乙醇。结果表明,表型转换是一个普遍的,高度变化的反应只发生在选择酿造菌株。然而,表现出切换行为的菌株改变了它们对外源浓度2-苯乙醇的成丝反应。
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3.30
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审稿时长
15 weeks
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