A combined treatment with progesterone, anti-inhibin serum, and equine chorionic gonadotropin improves number of ovulated oocytes in young C57BL/6J mice.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2023-08-11 DOI:10.1262/jrd.2023-036

Atsuko Kageyama, Mizuho Tsuchiya, Jumpei Terakawa, Junya Ito, Naomi Kashiwazaki

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Abstract

Superovulation procedures are routinely and widely used in mouse reproductive technology. Previous studies have shown that a large number of oocytes can be obtained from adult mice (> 10 weeks old) using a combined treatment with progesterone (P4) and anti-inhibin serum (AIS). However, these effects have not been fully investigated in young (4 weeks) C57BL/6J mice. Here, we found that a modified superovulation protocol (combined treatment with P4, AIS, eCG (equine chorionic gonadotropin), and hCG (human chorionic gonadotropin); P4D2-Ae-h) improved the number of oocytes compared to the control (eCG and hCG) (39.7 vs. 21.3 oocytes/mouse). After in vitro fertilization, pronuclear formation rates were 69.3% (P4D2-Ae-h group) and 66.2% (control group). After embryo transfer, 46.4% (116/250) of the embryos in the P4D2-Ae-h group successfully developed to term, which was comparable to the control group (42.9%; 123/287 embryos). In conclusion, our protocol (P4D2-Ae-h) was effective for superovulation in young C57BL/6J mice.

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黄体酮、抗抑制素血清和马绒毛膜促性腺激素联合治疗可提高幼年C57BL/6J小鼠的排卵卵母细胞数量。

超排卵程序是常规和广泛应用于小鼠生殖技术。先前的研究表明，使用黄体酮(P4)和抗抑制素血清(AIS)联合治疗，可以从成年小鼠(> 10周龄)获得大量卵母细胞。然而，这些影响尚未在幼龄(4周)C57BL/6J小鼠中得到充分研究。在这里，我们发现改良的超排卵方案(联合P4、AIS、eCG(马绒毛膜促性腺激素)和hCG(人绒毛膜促性腺激素);与对照组(eCG和hCG)相比，P4D2-Ae-h改善了卵母细胞数量(39.7对21.3个卵母细胞/小鼠)。体外受精后，原核形成率分别为69.3% (P4D2-Ae-h组)和66.2%(对照组)。胚胎移植后，P4D2-Ae-h组胚胎成功发育至足月的比例为46.4%(116/250)，与对照组(42.9%;123/287的胚胎)。总之，我们的方案(P4D2-Ae-h)对幼年C57BL/6J小鼠的超排卵有效。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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