Dendrobium Nobile Polysaccharides Attenuates Ferroptosis and Improves Cognitive Function in Vascular Dementia Rats.

IF 2.7 4区医学 Q2 CLINICAL NEUROLOGY American Journal of Alzheimers Disease and Other Dementias Pub Date : 2023-01-01 DOI:10.1177/15333175231185236

Min Ming, Weihua Hu, Gaosheng Xie, Jianping Chen, Ying Huang

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Abstract

Introduction: To investigate the neuroprotective effect and mechanism of Dendrobium nobile polysaccharide (DNP) on vascular dementia (VD) rats.

Methods: VD model rats were prepared by permanent ligation of bilateral common carotid arteries. Cognitive function was tested by morris water maze test, mitochondrial morphology and ultrastructure of hippocampal synapses were tested by transmission electron microscopy, GSH, xCT, GPx4, and PSD-95 expressions were tested by western blot and PCR.

Results: The number of platform crossing was significantly increased, and the escape latency was significantly shorter in DNP group. The expressions of GSH, xCT and GPx4 in the hippocampus were up-regulated in DNP group. Moreover, the synapses of DNP group were relatively intact and synaptic vesicles increased, the length of synaptic active zone and PSD thickness were significantly increased, and PSD-95 protein expression was significantly up-regulated compared VD group.

Conclusion: DNP may take a neuroprotective effect by inhibiting ferroptosis in VD.

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石斛多糖减轻血管性痴呆大鼠的铁下垂并改善其认知功能。

前言：探讨石斛多糖（DNP）对血管性痴呆（VD）大鼠的神经保护作用及其机制。方法：采用双侧颈总动脉永久结扎法制备VD模型大鼠。采用morris水迷宫法检测认知功能，透射电镜检测海马突触线粒体形态和超微结构，western blot和PCR检测GSH、xCT、GPx4和PSD-95的表达。DNP组大鼠海马GSH、xCT和GPx4表达上调。此外，与VD组相比，DNP组的突触相对完整，突触小泡增加，突触活动区长度和PSD厚度显著增加，PSD-95蛋白表达显著上调。结论：DNP可能通过抑制VD患者的脱铁性贫血而发挥神经保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American Journal of Alzheimers Disease and Other Dementias GERIATRICS & GERONTOLOGY-CLINICAL NEUROLOGY

CiteScore

5.40

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： American Journal of Alzheimer''s Disease and other Dementias® (AJADD) is for professionals on the frontlines of Alzheimer''s care, dementia, and clinical depression--especially physicians, nurses, psychiatrists, administrators, and other healthcare specialists who manage patients with dementias and their families. This journal is a member of the Committee on Publication Ethics (COPE).