Fabrication of an electrospun polycaprolactone substrate for colorimetric bioassays

IF 3 4区医学 Q3 ENGINEERING, BIOMEDICAL Biomedical Microdevices Pub Date : 2023-08-17 DOI:10.1007/s10544-023-00673-z

Chensong Xu, Gwenaël Bonfante, Jongho Park, Vincent Salles, Beomjoon Kim

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Abstract

Colorimetric assays rely on detecting colour changes to measure the concentration of target molecules. Paper substrates are commonly used for the detection of biomarkers due to their availability, porous structure, and capillarity. However, the morphological and mechanical properties of paper, such as fibre diameter, pore size, and tensile strength, cannot be easily tuned to meet the specific requirements of colorimetric sensors, including liquid capacity and reagent immobilisation. As an alternative to paper materials, biodegradable polymeric membranes made of electrospun polycaprolactone (PCL) fibres can provide various tunable properties related to fibre diameter and pore size.

We aimed to obtain a glucose sensor substrate for colorimetric sensing using electrospinning with PCL. A feeding solution was created by mixing PCL/chloroform and 3,3’,5’,5’-tetramethylbenzidine (TMB)/ethanol solutions. This solution was electrospun to fabricate a porous membrane composed of microfibres consist of PCL and TMB. The central area of the membrane was made hydrophilic through air plasma treatment, and it was subsequently functionalized with a solution containing glucose oxidase, horseradish peroxidase, and trehalose.

The sensing areas were evaluated by measuring colour changes in glucose solutions of varying concentrations. The oxidation reactions of glucose and TMB in sensor substrates were recorded and analysed to establish the correlation between different glucose concentrations and colour changes. For comparison, conventional paper substrates prepared with same parameters were evaluated alongside the electrospun PCL substrates. As a result, better immobilization of reagents and higher sensitivity of glucose were achieved with PCL substrates, indicating their potential usage as a new sensing substrate for bioassays.

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电纺聚己内酯底物比色生物测定的制备

比色法依靠检测颜色变化来测量目标分子的浓度。由于其可用性、多孔结构和毛细性，纸基通常用于生物标志物的检测。然而，纸张的形态和机械性能，如纤维直径、孔径和抗拉强度，不能轻易调整以满足比色传感器的特定要求，包括液体容量和试剂固定化。作为纸张材料的替代品，由静电纺聚己内酯(PCL)纤维制成的可生物降解聚合物膜可以提供与纤维直径和孔径有关的各种可调性能。我们的目的是获得葡萄糖传感器底物，用于静电纺丝与PCL的比色传感。将PCL/氯仿与3,3 '，5 '，5 ' -四甲基联苯胺(TMB)/乙醇溶液混合制成进料溶液。该溶液被静电纺丝制成由PCL和TMB组成的微纤维组成的多孔膜。通过空气等离子体处理使膜的中心区域亲水，随后用含有葡萄糖氧化酶、辣根过氧化物酶和海藻糖的溶液对其进行功能化。通过测量不同浓度葡萄糖溶液的颜色变化来评估感应区域。记录并分析了传感器底物中葡萄糖和TMB的氧化反应，以建立不同葡萄糖浓度与颜色变化之间的相关性。为了进行比较，将采用相同参数制备的传统纸质衬底与静电纺PCL衬底进行了比较。因此，PCL底物具有更好的固定试剂和更高的葡萄糖敏感性，表明其作为生物检测的新型传感底物的潜力。

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来源期刊

Biomedical Microdevices 工程技术-工程：生物医学

CiteScore

6.90

自引率

3.60%

发文量

审稿时长

6 months

期刊介绍： Biomedical Microdevices: BioMEMS and Biomedical Nanotechnology is an interdisciplinary periodical devoted to all aspects of research in the medical diagnostic and therapeutic applications of Micro-Electro-Mechanical Systems (BioMEMS) and nanotechnology for medicine and biology. General subjects of interest include the design, characterization, testing, modeling and clinical validation of microfabricated systems, and their integration on-chip and in larger functional units. The specific interests of the Journal include systems for neural stimulation and recording, bioseparation technologies such as nanofilters and electrophoretic equipment, miniaturized analytic and DNA identification systems, biosensors, and micro/nanotechnologies for cell and tissue research, tissue engineering, cell transplantation, and the controlled release of drugs and biological molecules. Contributions reporting on fundamental and applied investigations of the material science, biochemistry, and physics of biomedical microdevices and nanotechnology are encouraged. A non-exhaustive list of fields of interest includes: nanoparticle synthesis, characterization, and validation of therapeutic or imaging efficacy in animal models; biocompatibility; biochemical modification of microfabricated devices, with reference to non-specific protein adsorption, and the active immobilization and patterning of proteins on micro/nanofabricated surfaces; the dynamics of fluids in micro-and-nano-fabricated channels; the electromechanical and structural response of micro/nanofabricated systems; the interactions of microdevices with cells and tissues, including biocompatibility and biodegradation studies; variations in the characteristics of the systems as a function of the micro/nanofabrication parameters.