Molecular Characterization of Paederus Spp (Coleoptera: Staphylinidae, Paederinae) the Agent of Human Linear Dermatitis in the Caspian Sea Coast, North of Iran.

Pub Date : 2023-03-01 DOI:10.18502/jad.v17i1.13206

Abbas Heydari, Sinan Anlaş, Hasan Bakhshi, Mona Koosha, Nayyereh Choubdar, Somayeh Panahi-Moghadam, Mohammad Ali Oshaghi

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Abstract

Background: A combined morphological and molecular survey was performed to determine the agent of human linear dermatitis Paederus Fabricius, 1775 (Coleoptera: Staphylinidae, Paederinae) species composition in Mazandaran Province in the Caspian Sea coast in northern Iran, where most of linear dermatitis cases of the country occurred.

Methods: Altogether, 397 Paederus specimens were collected from May to August 2021 and classified using morphological characters and ITS2-rDNA sequence analysis.

Results: Morphological investigation revealed that all the specimens were Paederus fuscipes. ITS2 polymerase chain reaction (PCR) direct-sequences and the profiles of restriction fragment length polymorphism (RFLP) derived from digestion of PCR products by HinfI, HpaII, and SalI enzymes were identical confirming the morphological results, implying that all specimens belonged to a single taxon.

Conclusion: Paederus fuscipes (Fabricius, 1775) is considered the dominant taxon and responsible for linear dermatitis in Mazandaran Province. To our knowledge, we have provided the first molecular typing of Paederus beetles at the species level, suggesting that ITS2-rDNA characterization is an alternative tool for species discrimination of Paederus spp.

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伊朗北部里海沿岸人类线状皮炎病原拟蚊属(鞘翅目:葡萄球菌科，拟蚊科)的分子特征。

背景:对伊朗北部里海沿岸马赞达兰省(该国线状皮炎病例最多的地区)1775年人类线状皮炎病原进行形态学和分子调查，以确定其种类组成。方法:于2021年5月~ 8月采集拟蚊标本397份，利用形态学特征和ITS2-rDNA序列分析进行分类。结果:形态学调查显示所有标本均为梭形拟蚊。ITS2聚合酶链反应(PCR)直接序列与HinfI、HpaII和SalI酶切PCR产物的限制性内切片段长度多态性(RFLP)图谱一致，证实了形态学结果，表明所有标本属于单一分类单元。结论:fuscipes Paederus (Fabricius, 1775)被认为是马赞达兰省线状皮炎的优势分类群。据我们所知，我们首次在物种水平上提供了Paederus甲虫的分子分型，这表明ITS2-rDNA表征是Paederus物种区分的另一种工具。

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