Neocucurbitaria chlamydospora sp. nov.: A Novel Species of the Family Cucurbitariaceae Isolated from a Stink Bug in Korea.

IF 1.6 4区生物学 Q2 AGRONOMY Mycobiology Pub Date : 2023-05-12 eCollection Date: 2023-01-01 DOI:10.1080/12298093.2023.2203973

Soo-Min Hong, Kallol Das, Seong-Keun Lim, Sang Jae Suh, Seung-Yeol Lee, Hee-Young Jung

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Abstract

The fungal strain KNUF-22-18B, belonging to Cucurbitariaceae, was discovered from a stink bug (Hygia lativentris) during the investigation of insect microbiota in Chungnam Province, South Korea. The colonies of the strain KNUF-22-18B were wooly floccose, white to brown in the center on oatmeal agar (OA), and the colonies were buff, margin even, and colorless, reverse white to yellowish toward the center on malt extract agar (MEA). The strain KNUF-22-18B produced pycnidia after 60 days of culturing on potato dextrose agar, but pycnidia were not observed on OA. On the contrary, N. keratinophila CBS 121759^T abundantly formed superficial pycnidia on OA and MEA after a few days. The strain KNUF-22-18B produced chlamydospores subglobose to globose, mainly in the chain, with a small diameter of 4.4-8.8 μm. At the same time, N. keratinophila CBS 121759^T displayed a globose terminal with a diameter of 8-10 μm. A multilocus phylogeny using the internal transcribed spacer regions, 28S rDNA large subunit, β-tubulin, and RNA polymerase II large subunit genes further validated the uniqueness of the strain. The detailed description and illustration of the proposed species as Neocucurbitaria chlamydospora sp. nov. from Korea was strongly supported by molecular phylogeny.

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Neocucurbitaria chlamydospora sp：从韩国臭虫中分离出的葫芦科新物种。

菌株 KNUF-22-18B 属于葫芦科，是在韩国忠南道调查昆虫微生物群时从蝽中发现的。菌株 KNUF-22-18B 在燕麦琼脂（OA）上的菌落呈絮状，中心为白色至棕色；在麦芽提取物琼脂（MEA）上的菌落呈水绿色，边缘均匀，无色，向中心反转为白色至淡黄色。菌株 KNUF-22-18B 在马铃薯葡萄糖琼脂上培养 60 天后产生菌丝，但在 OA 上未观察到菌丝。相反，N. keratinophila CBS 121759T 几天后在 OA 和 MEA 上大量形成表层菌丝。菌株 KNUF-22-18B 产生的衣壳孢子近球形至球形，主要为链状，直径较小，为 4.4-8.8 μm。与此同时，N. keratinophila CBS 121759T 的顶端呈球状，直径为 8-10 μm。利用内部转录间隔区、28S rDNA 大亚基、β-微管蛋白和 RNA 聚合酶 II 大亚基基因进行的多焦点系统进化进一步验证了该菌株的独特性。分子系统进化论有力地支持了将该菌株详细描述和说明为来自韩国的 Neocucurbitaria chlamydospora sp.

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来源期刊

Mycobiology AGRONOMYMYCOLOGY-MYCOLOGY

CiteScore

3.90

自引率

5.30%

发文量

审稿时长

22 weeks

期刊介绍： Mycobiology is an international journal devoted to the publication of fundamental and applied investigations on all aspects of mycology and their traditional allies. It is published quarterly and is the official publication of the Korean Society of Mycology. Mycobiology publishes reports of basic research on fungi and fungus-like organisms, including yeasts, filamentous fungi, lichen fungi, oomycetes, moulds, and mushroom. Topics also include molecular and cellular biology, biochemistry, metabolism, developmental biology, environmental mycology, evolution, ecology, taxonomy and systematics, genetics/genomics, fungal pathogen and disease control, physiology, and industrial biotechnology using fungi.