ACE ID gene polymorphism contributes to chronic kidney disease progression but not NOS3 gene among Type 2 diabetes with nephropathy patients

Q3 Medicine Endocrine and Metabolic Science Pub Date : 2021-09-30 DOI:10.1016/j.endmts.2021.100100

Deepashree G A , Ramprasad E , Jayakumar M , Solomon F D Paul , Gnanasambandan R

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引用次数: 4

Abstract

Aim

Diabetic nephropathy (DN) is one of the major life-threatening complications of diabetes and it leads to end-stage renal disease. Altered angiotension converting enzyme and nitric oxide synthase are probably the cause of initiation and the progression of diabetic nephropathy. The present study aims to investigate the effect of ACE ID and NOS3 VNTR gene polymorphisms on the progression of chronic kidney disease among diabetic nephropathy.

Methods

253 DN patients and 104 controls were genotyped for ACE ID and NOS3 VNTR polymorphisms by following PCR-RFLP method. The diabetic nephropathy cases were divided into two groups based on CKD stages: 138 DN cases were at early stage (CKD1 to CKD3) and 115 DN cases were at advanced stage (CKD4 and CKD5). Association χ2 and univariate analysis were performed.

Results

A significant difference was found in genotype frequencies of ACE ID and NOS3 VNTR polymorphisms between the DN patients and the controls. On univariate analysis, the DD genotype of ACE gene was found to have a significant association with the advancement of CKD in DN (OR=0.37; 95 % CI=0.14–0.94; p=0.033).

Conclusions

The results suggest the association of ACE ID and NOS3 VNTR polymorphism with diabetic nephropathy in South Indian population. Furthermore, the present study evidences the association between DD genotype of ACE gene and advancement of CKD progression in DN.

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在2型糖尿病合并肾病患者中，ACE ID基因多态性对慢性肾病进展有影响，而NOS3基因无影响

糖尿病肾病(DN)是糖尿病的主要并发症之一，可导致终末期肾脏疾病。血管紧张转换酶和一氧化氮合酶的改变可能是糖尿病肾病发生和发展的原因。本研究旨在探讨ACE ID和NOS3 VNTR基因多态性对糖尿病肾病患者慢性肾病进展的影响。方法采用PCR-RFLP方法对253例DN患者和104例对照组进行ACE ID和NOS3 VNTR多态性基因分型。根据CKD分期将糖尿病肾病患者分为两组:早期肾病138例(CKD1 ~ CKD3)，晚期肾病115例(CKD4、CKD5)。进行关联χ2和单因素分析。结果肾病患者与对照组ACE ID和NOS3 VNTR多态性基因型频率差异有统计学意义。单因素分析发现，ACE基因DD基因型与肾病患者CKD的进展有显著相关性(OR=0.37;95% ci = 0.14-0.94;p = 0.033)。结论ACE ID和NOS3 VNTR多态性与南印度人群糖尿病肾病有关。此外，本研究还证实了ACE基因DD型与肾病患者CKD进展的相关性。

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