Whole genomes of deep-sea sponge-associated bacteria exhibit high novel natural product potential.

FEMS microbes Pub Date : 2023-02-22 eCollection Date: 2023-01-01 DOI:10.1093/femsmc/xtad005
Poppy J Hesketh-Best, Grant G January, Matthew J Koch, Philip J Warburton, Kerry L Howell, Mathew Upton
{"title":"Whole genomes of deep-sea sponge-associated bacteria exhibit high novel natural product potential.","authors":"Poppy J Hesketh-Best, Grant G January, Matthew J Koch, Philip J Warburton, Kerry L Howell, Mathew Upton","doi":"10.1093/femsmc/xtad005","DOIUrl":null,"url":null,"abstract":"<p><p>Global antimicrobial resistance is a health crisis that can change the face of modern medicine. Exploring diverse natural habitats for bacterially-derived novel antimicrobial compounds has historically been a successful strategy. The deep-sea presents an exciting opportunity for the cultivation of taxonomically novel organisms and exploring potentially chemically novel spaces. In this study, the draft genomes of 12 bacteria previously isolated from the deep-sea sponges <i>Phenomena carpenteri</i> and <i>Hertwigia</i> sp. are investigated for the diversity of specialized secondary metabolites. In addition, early data support the production of antibacterial inhibitory substances produced from a number of these strains, including activity against clinically relevant pathogens <i>Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa</i>, and <i>Staphylococcus aureus</i>. Draft whole-genomes are presented of 12 deep-sea isolates, which include four potentially novel strains: <i>Psychrobacter</i> sp. PP-21, <i>Streptomyces</i> sp. DK15, <i>Dietzia</i> sp. PP-33, and <i>Micrococcus</i> sp. M4NT. Across the 12 draft genomes, 138 biosynthetic gene clusters were detected, of which over half displayed less than 50% similarity to known BGCs, suggesting that these genomes present an exciting opportunity to elucidate novel secondary metabolites. Exploring bacterial isolates belonging to the phylum Actinomycetota, Pseudomonadota, and Bacillota from understudied deep-sea sponges provided opportunities to search for new chemical diversity of interest to those working in antibiotic discovery.</p>","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"4 ","pages":"xtad005"},"PeriodicalIF":0.0000,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a8/1d/xtad005.PMC10117722.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"FEMS microbes","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/femsmc/xtad005","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Global antimicrobial resistance is a health crisis that can change the face of modern medicine. Exploring diverse natural habitats for bacterially-derived novel antimicrobial compounds has historically been a successful strategy. The deep-sea presents an exciting opportunity for the cultivation of taxonomically novel organisms and exploring potentially chemically novel spaces. In this study, the draft genomes of 12 bacteria previously isolated from the deep-sea sponges Phenomena carpenteri and Hertwigia sp. are investigated for the diversity of specialized secondary metabolites. In addition, early data support the production of antibacterial inhibitory substances produced from a number of these strains, including activity against clinically relevant pathogens Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. Draft whole-genomes are presented of 12 deep-sea isolates, which include four potentially novel strains: Psychrobacter sp. PP-21, Streptomyces sp. DK15, Dietzia sp. PP-33, and Micrococcus sp. M4NT. Across the 12 draft genomes, 138 biosynthetic gene clusters were detected, of which over half displayed less than 50% similarity to known BGCs, suggesting that these genomes present an exciting opportunity to elucidate novel secondary metabolites. Exploring bacterial isolates belonging to the phylum Actinomycetota, Pseudomonadota, and Bacillota from understudied deep-sea sponges provided opportunities to search for new chemical diversity of interest to those working in antibiotic discovery.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
深海海绵相关细菌的全基因组显示出很高的新型天然产物潜力。
全球抗菌素耐药性是一场可以改变现代医学面貌的健康危机。为细菌衍生的新型抗菌化合物探索不同的自然栖息地历来是一种成功的策略。深海为培育分类上的新生物和探索潜在的化学新空间提供了一个令人兴奋的机会。在这项研究中,研究了以前从深海海绵中分离出来的12种细菌的基因组草案,研究了专门的次级代谢物的多样性。此外,早期数据支持从这些菌株中生产抗菌抑制物质,包括抗临床相关病原体鲍曼不动杆菌、大肠杆菌、肺炎克雷伯菌、铜绿假单胞菌和金黄色葡萄球菌的活性。本文报道了12个深海分离株的全基因组草图,其中包括4个潜在的新菌株:Psychrobacter sp. PP-21、Streptomyces sp. DK15、Dietzia sp. PP-33和Micrococcus sp. M4NT。在12个基因组草案中,检测到138个生物合成基因簇,其中一半以上与已知bgc的相似性低于50%,这表明这些基因组为阐明新的次级代谢物提供了令人兴奋的机会。从深海海绵中探索属于放线菌门、假单胞菌门和芽孢杆菌门的细菌分离物,为那些从事抗生素发现工作的人提供了寻找新的化学多样性的机会。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
3.30
自引率
0.00%
发文量
0
审稿时长
15 weeks
期刊最新文献
Evaluating the impact of redox potential on the growth capacity of anaerobic gut fungi. Contact with young children is a major risk factor for pneumococcal colonization in older adults. Trivalent immunization with metal-binding proteins confers protection against enterococci in a mouse infection model. Arginine impacts aggregation, biofilm formation, and antibiotic susceptibility in Enterococcus faecalis. Pandemic storytelling and student engagement: how students imagined pandemics before COVID-19 pandemic.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1