Investigation of Properties of the Mitochondrial Permeability Transition Pore Using Whole-Mitoplast Patch-Clamp Technique.

IF 2.6 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY DNA and cell biology Pub Date : 2023-08-01 Epub Date: 2023-06-13 DOI:10.1089/dna.2023.0171
Maria A Neginskaya, Evgeny V Pavlov
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Abstract

The mitochondrial permeability transition pore (mPTP) is a channel in the mitochondrial inner membrane that is activated by excessive calcium uptake. In this study, we used a whole-mitoplast patch-clamp approach to investigate the ionic currents associated with mPTP at the level of the whole single mitochondrion. The whole-mitoplast conductance was at the level of 5 to 7 nS, which is consistent with the presence of three to six single mPTP channels per mitochondrion. We found that mPTP currents are voltage dependent and inactivate at negative potential. The currents were inhibited by cyclosporine A and adenosine diphosphate. When mPTP was induced by oxidative stress, currents were partially blocked by the adenine nucleotide translocase inhibitor bongkrekic acid. Our data suggest that the whole-mitoplast patch-clamp approach is a useful method for investigating the biophysical properties and regulation of the mPTP.

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利用全线粒体膜片钳技术研究线粒体通透性转换孔的特性
线粒体通透性转换孔(mPTP)是线粒体内膜上的一个通道,会被过量的钙吸收激活。在这项研究中,我们采用全线粒体膜片钳方法,在整个单个线粒体水平上研究了与 mPTP 相关的离子电流。整个线粒体的电导水平为 5 到 7 nS,这与每个线粒体存在 3 到 6 个 mPTP 通道相一致。我们发现,mPTP 电流与电压有关,在负电位时失活。环孢素 A 和二磷酸腺苷可抑制电流。当氧化应激诱导 mPTP 时,腺嘌呤核苷酸转运酶抑制剂邦克瑞克酸可部分阻断电流。我们的数据表明,全丝质体膜片钳方法是研究 mPTP 生物物理特性和调控的有用方法。
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来源期刊
DNA and cell biology
DNA and cell biology 生物-生化与分子生物学
CiteScore
6.60
自引率
0.00%
发文量
93
审稿时长
1.5 months
期刊介绍: DNA and Cell Biology delivers authoritative, peer-reviewed research on all aspects of molecular and cellular biology, with a unique focus on combining mechanistic and clinical studies to drive the field forward. DNA and Cell Biology coverage includes: Gene Structure, Function, and Regulation Gene regulation Molecular mechanisms of cell activation Mechanisms of transcriptional, translational, or epigenetic control of gene expression Molecular Medicine Molecular pathogenesis Genetic approaches to cancer and autoimmune diseases Translational studies in cell and molecular biology Cellular Organelles Autophagy Apoptosis P bodies Peroxisosomes Protein Biosynthesis and Degradation Regulation of protein synthesis Post-translational modifications Control of degradation Cell-Autonomous Inflammation and Host Cell Response to Infection Responses to cytokines and other physiological mediators Evasive pathways of pathogens.
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