Evaluation of mRNA Expression of CD244 and Its Adapter Molecules in CD8+ T Cells in Acute Leukemia.

Q2 Biochemistry, Genetics and Molecular Biology Iranian Biomedical Journal Pub Date : 2023-07-01 DOI:10.52547/ibj.3843

Maryam Mohammadi, Hossein Asgarian-Omran, Ahmad Najafi, Reza Valadan, Hossein Karami, Mohammad Naderisoraki, Ehsan Zaboli, Mohammad Eslami, Mohsen Tehrani

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Abstract

Background: This study investigated the role of the immune-checkpoint receptor (ICR), CD244, and its adapter molecules, in CD8+ T cells in acute leukemia.

Methods: Blood samples were obtained from 21 acute lymphoblastic leukemia (ALL) and 6 acute myeloid leukemia (AML) patients and 20 control subjects. Relative gene expression of CD244, immune receptor tyrosine-based switch motif-associated protein (SA), EWS/FLI1-activated transcript 2 (EAT-2), and LncRNA-GSTT1-AS1 were evaluated using quantitative reverse transcription polymerase chain reaction.

Results: Expression of CD244, SAP, and EAT-2 were significantly lower in CD8+ T cells from ALL patients than those from control subjects. Interestingly, the expression of SAP was much lower than that of CD244, indicating a lower ratio of SAP to CD244. Also, SAP expression was significantly lower in AML patients compared to the control group. Expression of LncRNA-GSTT1-AS1 showed no significant difference in ALL and AML patients compared to control subjects.

Conclusion: The low SAP/CD244 expression ratio in CD8+ T cells in ALL suggests an inhibitory role for CD244 in ALL.

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CD244及其衔接分子在急性白血病CD8+T细胞中mRNA表达的评估。

背景：本研究探讨了免疫检查点受体（ICR）CD244及其衔接分子在急性白血病CD8+T细胞中的作用。方法：采集21例急性淋巴细胞白血病（ALL）、6例急性髓系白血病（AML）患者和20例对照者的血液样本。使用定量逆转录聚合酶链反应评估CD244、基于免疫受体酪氨酸的开关基序相关蛋白（SA）、EWS/FLI1激活的转录物2（EAT-2）和LncRNA-GSTT1-AS1的相对基因表达。结果：ALL患者CD8+T细胞中CD244、SAP和EAT-2的表达显著低于对照组。有趣的是，SAP的表达远低于CD244，表明SAP与CD244的比率较低。此外，与对照组相比，AML患者的SAP表达显著降低。与对照受试者相比，ALL和AML患者中LncRNA-GSTT1-AS1的表达没有显著差异。结论：急性淋巴细胞白血病CD8+T细胞中SAP/CD244的低表达率提示CD244在急性淋巴细胞白血病中具有抑制作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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