Protective and Therapeutic Role of Ginkgo Biloba Extract Through TRPV1 Channels in an in Vitro Alzheimer's Disease Model.

IF 1 4区医学 Q4 CLINICAL NEUROLOGY Noropsikiyatri Arsivi-Archives of Neuropsychiatry Pub Date : 2023-01-01 DOI:10.29399/npa.28166

Ahmet Özşimşek, İshak Suat Övey, Ertan Karaçay, Burak Yuluğ

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Abstract

Introduction: The effect of Ginkgo biloba (GB) on mitochondria-dependent TRPV1 ion channels in neuroblastoma cells was investigated by creating an Alzheimer's disease (AD) model.

Methods: Okadaic acid was applied on SH-SY5Y cells to create an AD model. After cellular differentiation, the study was organized with the seven main groups, examining the effect of GB on calcium depended TRPV1 channels in neuroblastoma cells AD, has been established in vitro.

Results: The higher Ca²+ concentration was detected in the GB+AD, AD and AD+GB groups when compared with the control (p<0.001). The Ca²+ level was lower in GB+AD and AD+GB groups than in the AD group (p<0.001). Also, cytosolic Ca²+ concentration was lower in the GB+AD than in the AD+GB group (p<0.05), the apoptosis and intracellular reactive oxygen species (ROS) values were higher in the GB+AD, AD and AD+GB groups than in the control (p<0.001). The apoptosis and intracellular ROS values were higher in AD group than in the GB+AD and AD+GB group (p<0.001) and the apoptosis level was higher in AD+GB group than GB+AD group (p<0.001) and the mitochondrial depolarization, caspase 3 and caspase 9 levels were higher in the GB+AD, AD and AD+GB groups when compared to the control group (p<0.001). Also, the values were lower in the GB+AD group, AD group and AD+GB groups when compared with the GB+AD+capsazepine group, AD+capsazepine group and AD+GB+capsazepine respectively (p<0.001).

Conclusion: These results show us that GB has a protective effect besides its therapeutic effect in Alzheimer's disease via TRPV1 channel.

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银杏叶提取物通过TRPV1通道对阿尔茨海默病体外模型的保护和治疗作用

简介:通过建立阿尔茨海默病(AD)模型，研究银杏叶(GB)对神经母细胞瘤细胞线粒体依赖性TRPV1离子通道的影响。方法:采用冈田酸作用于SH-SY5Y细胞，建立AD模型。细胞分化后，本研究分为7个主要组，在体外建立了GB对AD神经母细胞瘤细胞钙依赖性TRPV1通道的影响。结果:与对照组相比，GB+AD组、AD组和AD+GB组Ca2+浓度较高(GB+AD组和AD+GB组p2+水平低于AD组)(GB+AD组p2+浓度低于AD+GB组(p)结论:这些结果表明，GB通过TRPV1通道对阿尔茨海默病具有保护作用，同时具有治疗作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Noropsikiyatri Arsivi-Archives of Neuropsychiatry CLINICAL NEUROLOGY-

CiteScore

1.70

自引率

9.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Archives of Neuropsychiatry (Arch Neuropsychiatry) is the official journal of the Turkish Neuropsychiatric Society. It is published quarterly, and four editions annually constitute a volume. Archives of Neuropsychiatry is a peer reviewed scientific journal that publishes articles on psychiatry, neurology, and behavioural sciences. Both clinical and basic science contributions are welcomed. Submissions that address topics in the interface of neurology and psychiatry are encouraged. The content covers original research articles, reviews, letters to the editor, and case reports.