Large scale rice germplasm screening for identification of novel brown planthopper resistance sources.

IF 2.6 3区农林科学 Q1 AGRONOMY Molecular Breeding Pub Date : 2023-08-28 eCollection Date: 2023-09-01 DOI:10.1007/s11032-023-01416-x

Ke Yang, Hongmei Liu, Weihua Jiang, Yinxia Hu, Zhiyang Zhou, Xin An, Si Miao, Yushi Qin, Bo Du, Lili Zhu, Guangcun He, Rongzhi Chen

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Abstract

Rice (Oryza sativa L.) is a staple food crop globally. Brown planthopper (Nilaparvata lugens Stål, BPH) is the most destructive insect that threatens rice production annually. More than 40 BPH resistance genes have been identified so far, which provide valuable gene resources for marker-assisted breeding against BPH. However, it is still urgent to evaluate rice germplasms and to explore more new wide-spectrum BPH resistance genes to combat newly occurring virulent BPH populations. To this end, 560 germplasm accessions were collected from the International Rice Research Institute (IRRI), and their resistance to current BPH population of China was examined. A total of 105 highly resistant materials were identified. Molecular screening of BPH resistance genes in these rice germplasms was conducted by developing specific functional molecular markers of eight cloned resistance genes. Twenty-three resistant germplasms were found to contain none of the 8 cloned BPH resistance genes. These accessions also exhibited a variety of resistance mechanisms as indicated by an improved insect weight gain (WG) method, suggesting the existence of new resistance genes. One new BPH resistance gene, Bph44(t), was identified in rice accession IRGC 15344 and preliminarily mapped to a 0-2 Mb region on chromosome 4. This study systematically sorted out the corresponding relationships between BPH resistance genes and germplasm resources using a functional molecular marker system. Newly explored resistant germplasms will provide valualble donors for the identification of new resistance genes and BPH resistance breeding programs.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-023-01416-x.

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大规模水稻种质筛选鉴定褐飞虱新抗性来源。

水稻（Oryza sativa L.）是全球的主要粮食作物。褐飞虱（Nilaparvata lugens Stål，BPH）是每年威胁水稻生产的最具破坏性的昆虫。到目前为止，已经鉴定出40多个BPH抗性基因，为BPH的标记辅助育种提供了宝贵的基因资源。然而，评估水稻种质资源和探索更多新的广谱抗褐飞虱基因以对抗新出现的强褐飞虱种群仍然是当务之急。为此，从国际水稻研究所（IRRI）收集了560份种质资源，并对其对中国现有褐飞虱种群的抗性进行了检测。共鉴定出105种高抗性材料。通过对8个克隆的水稻抗褐飞虱基因进行特异性功能分子标记的构建，对这些水稻种质中的褐飞虱抗性基因进行了分子筛选。23份抗性种质中未发现8个克隆的BPH抗性基因。这些材料还表现出多种抗性机制，如改进的昆虫增重（WG）方法所示，表明存在新的抗性基因。在水稻登录号IRGC 15344中鉴定出一个新的抗褐飞虱基因Bph44（t），并初步定位到4号染色体上的0-2Mb区域。本研究利用功能分子标记系统，系统梳理了褐飞虱抗性基因与种质资源的对应关系。新发现的抗性种质将为鉴定新的抗性基因和BPH抗性育种计划提供有价值的供体。补充信息：在线版本包含补充材料，可访问10.1007/s11032-023-01416-x。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.