High Prevalence of Panton-valentine Leukocidin-encoding Genes in Methicillin-resistant Staphylococcus aureus Isolated from Inpatients with Invasive Infections at a University Hospital in Southern Brazil.

Q3 Pharmacology, Toxicology and Pharmaceutics Infectious disorders drug targets Pub Date : 2023-01-01 DOI:10.2174/1871526522666220823164600

Raquel Soares da Silva, Felipe Crepaldi Duarte, Tiago Danelli, Anna Paula Silva Olak, Gerusa Luciana Gomes Magalhães, Marsileni Pelisson, Stefani Lino Cardim, Guilherme Bartolomeu Gonçalves, Eliana Carolina Vespero, Eliandro Reis Tavares, Lucy Megumi Yamauchi, Marcia Regina Eches Perugini, Sueli Fumie Yamada-Ogatta

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引用次数: 1

Abstract

Background: Staphylococcus aureus is a major cause of a wide diversity of infections in humans, and the expression of Panton-Valentine Leukocidin (PVL) has been associated with severe clinical syndromes.

Objectives: The present study aimed to investigate the prevalence of PVL-encoding genes in S. aureus isolated from clinical samples of inpatients with invasive infections in a teaching hospital in Southern Brazil. Furthermore, phenotypic and genotypic characteristics of bacterial isolates were analyzed.

Methods: A total of 98 S. aureus isolates recovered from different body sites were characterized according to their antimicrobial susceptibility profile, methicillin-resistance and SCCmec typing, genetic relatedness and occurrence of virulence-encoding genes, such as icaA, lukS-PV/lukF-PV, and tst.

Results: Sixty-eight (69.4%) isolates were classified as methicillin-resistant, and among them, four (5.9%) did not harbor the mecA gene. The mecA-harboring methicillin-resistant S. aureus (MRSA) isolates were grouped into SCCmec types I (6.3%), II (64.1%), III (6.3%), IV (15.6%), V (4.7%), and VI (1.6%). One isolate (1.6%) was classified as non-typeable (NT). Seventy isolates (71.4%) were classified as multidrug-resistant. The overall prevalence of virulence-encoding genes was as follows: icaA, 99.0%; tst, 27.5%; and lukS-PV/lukF-PV, 50.0%. The presence of tst gene was significantly higher (p < 0.001) in methicillin-susceptible S. aureus (MSSA) compared to MRSA isolates.

Conclusion: The present study reports a high prevalence of multidrug-resistant S. aureus harboring lukS-PV/lukF-PV and tst genes in invasive infections. The continuous monitoring of the antimicrobial susceptibility profile and virulence of S. aureus is an important measure for the control of infections caused by this bacterium.

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巴西南部一所大学医院侵袭性感染住院患者中分离出的耐甲氧西林金黄色葡萄球菌中潘通-瓦伦丁白细胞编码基因的高流行率

背景:金黄色葡萄球菌是人类多种感染的主要原因，Panton-Valentine Leukocidin (PVL)的表达与严重的临床综合征有关。目的:本研究旨在调查从巴西南部一家教学医院侵袭性感染住院患者临床样本中分离的金黄色葡萄球菌中pvl编码基因的流行情况。此外，还分析了菌株的表型和基因型特征。方法:对98株不同部位金黄色葡萄球菌进行药敏、耐甲氧西林、SCCmec分型、icaA、lukS-PV/lukF-PV、tst等毒力编码基因的遗传相关性和发生情况等分析。结果:耐甲氧西林菌株68株(69.4%)，其中不含mecA基因4株(5.9%)。携带甲氧西林的耐甲氧西林金黄色葡萄球菌(MRSA)分为SCCmec I型(6.3%)、II型(64.1%)、III型(6.3%)、IV型(15.6%)、V型(4.7%)和VI型(1.6%)。1株(1.6%)为不可分型(NT)。70株(71.4%)为多重耐药菌株。毒力编码基因总体流行率为:icaA, 99.0%;结核菌素,27.5%;lukS-PV/lukF-PV为50.0%。与MRSA分离株相比，甲氧西林敏感金黄色葡萄球菌(MSSA)中tst基因的存在显著增加(p < 0.001)。结论:本研究报告了侵袭性感染中携带lukS-PV/lukF-PV和test基因的多重耐药金黄色葡萄球菌的高发率。持续监测金黄色葡萄球菌的药敏特征和毒力是控制金黄色葡萄球菌感染的重要措施。

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来源期刊

Infectious disorders drug targets Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

3.10

自引率

0.00%

发文量

123

期刊介绍： Infectious Disorders - Drug Targets aims to cover all the latest and outstanding developments on the medicinal chemistry, pharmacology, molecular biology, genomics and biochemistry of contemporary molecular targets involved in infectious disorders e.g. disease specific proteins, receptors, enzymes, genes. Each issue of the journal contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics on drug targets involved in infectious disorders. As the discovery, identification, characterization and validation of novel human drug targets for anti-infective drug discovery continues to grow, this journal will be essential reading for all pharmaceutical scientists involved in drug discovery and development.