Extended-Spectrum β-Lactamase-Producing Escherichia coli and Virulence Genes in Pediatric Patients with Health-Care Urinary Tract Infections.

Q3 Pharmacology, Toxicology and Pharmaceutics Infectious disorders drug targets Pub Date : 2023-01-01 DOI:10.2174/1871526523666221104150123

Mohamed Anies Rizk, Maysaa El Sayed Zaki, Hanan Abdelfattah Abdelmohsen Mohamed, Dina Mohammed Abdelhady Mahmood, Karim Monatasser

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Abstract

Introduction: Healthcare-associated urinary tract infection (UTI) represents a significant health problem, especially in infants and young children. The most common pathogen associated with this infection is Escherichia coli (E. coli).

Objective: The present study aimed to detect the frequency of virulence genes among clinical isolates of E. coli isolated from healthcare-associated urinary tract infections in children and the correlation between these virulence genes and the presence of the bla_CTX gene.

Methods: The study included one hundred clinical isolates of E. coli isolated from healthcareassociated urinary tract infections in children in intensive care units. The isolates were subjected to antibiotics sensitivity by disc diffusion method and detection of extended-spectrum beta-lactamase by double disc diffusion method. In addition, multiplex polymerase chain reaction (PCR) was used to detect some virulence genes, and PCR was used to detect the bla_CTX-M gene.

Results: E. coli producing ESBL by double discs method was identified in 74 isolates. bla_CTX-M gene detection by PCR was identified among 38 isolates representing 51.4% of ESBL-producing E. coli. There was a significant association between ESBL and bla_CTX-M Gene, P = 0.0001. The frequency of the studied virulence genes by multiplex PCR in the isolated E. coli was 66% for the Fim gene, 75% for the Aer gene, 68% for the FliC gene, 53% for each of IucD gene and Usp gene, 40% for pap gene, 35% for each of AFA and ironN genes and 17% for sfa gene. None of the isolated E. coli had the Cdt gene. There was a significant association between the presence of the FimH gene (P = 0.0001), Pap gene (P = 0.05), sfa (P = 0.026), Afa gene (P = 0.018), and aer gene (P = 0.035) and the presence of the bla_CTX-M gene in the isolated E. coli.

Conclusion: The present study highlights the presence of virulence genes and bla_CTX-M gene in uropathogenic E. coli isolated from pediatric patients with healthcare-associated urinary tract infections. There was an association between the bla_CTX-M gene and virulence genes FimH, pap, sfa, Afa, and aer. Various distributions of the studied genes with a high frequency of fimbria are flic genes. Moreover, the ESBL had high frequency in E. coli with the presence of bla_CTX-M in about one-third of the isolates.

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保健尿路感染患儿产β-内酰胺酶大肠杆菌及其毒力基因

导读:医疗保健相关的尿路感染(UTI)是一个重要的健康问题，特别是在婴幼儿中。与这种感染相关的最常见病原体是大肠杆菌。目的:本研究旨在检测儿童卫生保健相关尿路感染大肠杆菌临床分离株中毒力基因的频率，以及这些毒力基因与blaCTX基因存在的相关性。方法:本研究纳入了从重症监护病房中卫生保健相关尿路感染的儿童中分离出的100株大肠杆菌。采用圆盘扩散法对分离菌株进行药敏试验，采用双圆盘扩散法检测扩展谱β -内酰胺酶。此外，采用多重聚合酶链反应(PCR)检测部分毒力基因，并采用PCR检测blaCTX-M基因。结果:在74株分离株中鉴定出双片法产ESBL的大肠杆菌。在38株产esbl的大肠杆菌中检测到blaCTX-M基因，占51.4%。ESBL与blaCTX-M基因有显著相关性，P = 0.0001。在分离的大肠杆菌中，多重PCR所研究的毒力基因频率分别为:薄膜基因66%、Aer基因75%、FliC基因68%、IucD基因和Usp基因各53%、pap基因40%、AFA基因和铁蛋白基因各35%、sfa基因17%。分离的大肠杆菌中没有Cdt基因。分离的大肠杆菌中FimH基因(P = 0.0001)、Pap基因(P = 0.05)、sfa基因(P = 0.026)、Afa基因(P = 0.018)和aer基因(P = 0.035)的存在与blaCTX-M基因存在显著相关。结论:本研究强调了从儿童卫生保健相关尿路感染患者分离的尿路致病性大肠杆菌中存在毒力基因和blaCTX-M基因。blaCTX-M基因与毒力基因FimH、pap、sfa、Afa和aer存在相关性。所研究基因的各种分布与高频率的被毛是flic基因。此外，ESBL在大肠杆菌中频率较高，约三分之一的分离株中存在blaCTX-M。

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来源期刊

Infectious disorders drug targets Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

3.10

自引率

0.00%

发文量

123

期刊介绍： Infectious Disorders - Drug Targets aims to cover all the latest and outstanding developments on the medicinal chemistry, pharmacology, molecular biology, genomics and biochemistry of contemporary molecular targets involved in infectious disorders e.g. disease specific proteins, receptors, enzymes, genes. Each issue of the journal contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics on drug targets involved in infectious disorders. As the discovery, identification, characterization and validation of novel human drug targets for anti-infective drug discovery continues to grow, this journal will be essential reading for all pharmaceutical scientists involved in drug discovery and development.