Recognition of 7 genes signature (Cirrhosis Risk Score) in the diagnosed non-responders to DAAs therapy by intra-PBMCs nested HCV RNA PCR.

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal, genetic engineering & biotechnology Pub Date : 2023-08-30 DOI:10.1186/s43141-023-00544-3
Al-Shazly Gaber Mohamed Galal, Reham M Dawood, Mostafa K El Awady, Yasser Mohamed Mohamed El-Dessouky, Mohamed Mahmoud Abdel-Halim Mahmoud, Mohamed Darwish Ahmed Abd Alla
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Abstract

Background and aims: Predictors of chronic HCV response to oral antiviral therapy (OAT) are related to host genetic variations. Single nucleotide polymorphisms (SNP) and alleles variations of host genes in association with hepatic fibro-cirrhotic changes have a distinct role in OAT outcomes. The current research evaluated the association of Cirrhosis-Risk-Scores (CRS) values, based on the correlation of seven genes signature-SNPs, with sonographic liver parenchymal changes in determining OAT outcomes.

Methods: All study subjects (n = 54) were recruited three months after completing OAT and classified into three groups. Group I (n = 21) had negative HCV PCR, group II (n = 17) showed positive solitary intra-PBMCs HCV infection, and group III(n = 16) was serum HCV RNA PCR-positive. All study-population were subjected to examination by hepatic-ultrasound (US), FIB-4-scoring, and screening for 7 gene-signature that addressed CRS values as low, intermediate, and high depending on gene SNPs identification.

Results: Group I showed a significant association with low CRS values compared to other groups (P < 0.001). Solitary intra- PBMCs HCV infection in group II was significantly combined with intermediate CRS values in comparison to groups I and III (P < 0.001). The high CRS values were significantly found in group III when compared to groups I and II (P < 0.01). On US imaging, low CRS values were common in normally appeared hepatic parenchyma (P < 0.001) and high CRS values were frequent in coarse-liver (P < 0.001), while bright-liver-tissues appearance was mainly detected in the intermediate CRS category (P = 0.09). On FIB-4 scoring, high CRS value were associated with hepatic fibro-cirrhosis compared to intermediate (P < 0.001) and low (P = 0.08) CRS-categories.

Conclusion: The current study concluded the association of (a) high CRS values with coarse liver in viral-RNA serologic relapse, (b) low CRS values with normal liver tissues in sustained virologic response (SVR), (c) intermediate CRS values with bright liver in solitary PBMCs relapse.

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pbmcs内嵌套HCV RNA PCR对DAAs治疗无应答者7个基因特征(肝硬化风险评分)的识别
背景和目的:慢性丙型肝炎病毒对口服抗病毒治疗(OAT)反应的预测因素与宿主遗传变异有关。与肝纤维化变化相关的宿主基因的单核苷酸多态性(SNP)和等位基因变异在OAT结果中具有明显的作用。目前的研究评估了肝硬化风险评分(CRS)值的相关性,基于七个基因特征snp的相关性,在确定OAT结果的超声肝实质改变。方法:所有研究对象(n = 54)在完成OAT后3个月招募,分为3组。ⅰ组(n = 21) HCV PCR阴性,ⅱ组(n = 17)单发pbmc内HCV感染阳性,ⅲ组(n = 16)血清HCV RNA PCR阳性。所有研究人群均接受肝超声(US)检查,fib -4评分,并筛选7个基因特征,根据基因snp鉴定将CRS值划分为低、中、高。结果:与其他组相比,组1与低CRS值显著相关(P结论:目前的研究得出:(a)高CRS值与病毒- rna血清学复发的粗肝有关,(b)低CRS值与持续病毒学反应(SVR)的正常肝组织有关,(c)中等CRS值与孤立性PBMCs复发的亮肝有关。
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