Increased tube formation and up-regulation of FGFR3 mRNA expression in microvascular endothelial cell by exosomes derived from SW480-7.

IF 0.6 4区医学 Q4 PATHOLOGY Malaysian Journal of Pathology Pub Date : 2023-08-01

C T Ng, W K Yip, N Mohtarrudin, M F Jabar, H F Seow

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引用次数: 0

Abstract

Introduction: Extracellular vesicles (exosome-like vesicles) are small membrane vesicles ranging from 20-200nm in size that are released by various cells into the extracellular space. These extracellular vesicles play a major role in cell-to-cell communication and contain materials, such as proteins, mRNAs, microRNAs (miRNAs) and long non-coding RNAs (lncRNAs). The effect of exosomes derived from an invasive colon cancer cell line on angiogenesis is unclear. Hence, the aim of this study is to investigate the effect of exosomes derived from an invasive colon cancer cell line on angiogenesis of endothelial cells.

Materials and methods: In the present study, the exosomes from the cell culture supernatants of an invasive colon cancer cell line SW480-7 were characterised. The effect on tube formation and expression of angiogenic genes in a microvascular endothelial cell, telomerase-immortalised microvascular endothelial cell (TIME) was examined after co-cultured with exosomes secreted from SW480-7.

Results: Zetasizer result showed average diameter of exosomes derived from SW480-7 was 246.2 nm and morphological analysis showed the size of majority of exosomes were less than 200 nm. Results showed that exosomes derived from SW480-7 increased tube formation and up-regulated FGFR3 mRNA expression in TIME.

Conclusion: Our findings suggest that exosomes derived from SW480-7 increased tube formation and up-regulated expression of FGFR3 mRNA in TIME.

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SW480-7衍生的外泌体增加微血管内皮细胞的管状形成和上调FGFR3 mRNA表达。

细胞外囊泡(外泌体样囊泡)是由各种细胞释放到细胞外空间的小的膜囊泡，大小在20-200nm之间。这些细胞外囊泡在细胞间通讯中起着重要作用，并含有蛋白质、mrna、microRNAs (miRNAs)和长链非编码rna (lncRNAs)等物质。来自侵袭性结肠癌细胞系的外泌体对血管生成的影响尚不清楚。因此，本研究的目的是研究来自侵袭性结肠癌细胞系的外泌体对内皮细胞血管生成的影响。材料和方法:本研究对侵袭性结肠癌细胞系SW480-7细胞培养上清中的外泌体进行了表征。研究了端粒酶永生化微血管内皮细胞(TIME)与SW480-7分泌的外泌体共培养后对血管生成基因表达和管状形成的影响。结果:Zetasizer结果显示SW480-7衍生的外泌体平均直径为246.2 nm，形态学分析显示大多数外泌体的大小小于200 nm。结果显示SW480-7衍生的外泌体增加了小管的形成，上调了FGFR3 mRNA的表达。结论:我们的研究结果表明，SW480-7衍生的外泌体增加了TIME中小管的形成并上调了FGFR3 mRNA的表达。

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来源期刊

Malaysian Journal of Pathology PATHOLOGY-

CiteScore

3.60

自引率

5.60%

发文量

期刊介绍： The Malaysian Journal of Pathology is the official journal of the College of Pathologists, Academy of Medicine Malaysia. The primary purpose of The Journal is to publish the results of study and research in Pathology, especially those that have particular relevance to human disease occurring in Malaysia and other countries in this region. The term PATHOLOGY will be interpreted in its broadest sense to include Chemical Pathology, Cytology, Experimental Pathology, Forensic Pathology, Haematology, Histopathology, Immunology, Medical Microbiology and Parasitology. The Journal aims to bring under one cover publications of regional interest embracing the various sub-specialities of Pathology. It is expected that the articles published would be of value not only to pathologists, but also to medical practitioners in search of a scientific basis for the problems encountered in their practice, and to those with an interest in diseases which occur in the tropics.