Electroacupuncture alleviates cognitive dysfunction and neuronal pyroptosis in septic mice.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2023-08-01 DOI:10.1177/09645284221117847

Yan Li, Zhaoying Li, Fujuan He, Chenguang Qin, Rui Fan, Fangxiang Zhang, Bin Wang

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引用次数: 3

Abstract

Background: Sepsis is defined as organ dysfunction caused by an uncontrolled response to infection and is followed by a high incidence of cognitive dysfunction, which can severely affect patients' quality of life. Previous studies have suggested that electroacupuncture (EA) is protective against sepsis-associated cognitive dysfunction and that pyroptosis plays a vital role in cognitive function. The aim of this study was to investigate the effect of EA on cognition and neuronal pyroptosis in a mouse model of sepsis.

Methods: Sepsis was induced by cecal ligation and puncture (CLP) surgery. Mice were randomly divided into three groups (control, CLP and CLP + EA). EA was performed at bilateral ST36 for three consecutive days after the surgery. The 7-day survival rate of each group was observed on the seventh day after the surgery. The Morris water maze (MWM) was used to test cognitive function from the 8th to 12th day after the surgery. We used transmission electron microscopy (TEM) and transferase dUTP nick-end labeling (TUNEL) staining to determine the structural integrity of hippocampal neuronal membranes and the number of surviving neurons in the hippocampal tissues, respectively. Expression of nucleotide-binding domain-like receptor protein 1 (NLRP1), caspase-1 and gasdermin-D (GSDM D) in hippocampal CA1 neurons was detected by Western blotting and real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), and caspase-1 concentrations were measured by enzyme-linked immunosorbent assay.

Results: Compared with the CLP group, 7-day survival rates and cognitive function were significantly improved in the CLP + EA group. After EA treatment, the integrity of the hippocampal CA1 neuronal membrane and mortality of hippocampal neurons were significantly decreased, and expression of NLRP1, caspase-1 and GSDM D was downregulated.

Conclusion: EA can alleviate cognitive dysfunction and neuronal pyroptosis in septic mice.

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电针可减轻脓毒症小鼠的认知功能障碍和神经元焦亡。

背景:脓毒症被定义为由于对感染的反应不受控制而引起的器官功能障碍，并伴有高发的认知功能障碍，严重影响患者的生活质量。以往的研究表明，电针(EA)对脓毒症相关的认知功能障碍有保护作用，焦亡在认知功能中起着至关重要的作用。本研究的目的是研究EA对脓毒症小鼠模型的认知和神经元焦亡的影响。方法:采用盲肠结扎穿刺术(CLP)诱导脓毒症。将小鼠随机分为对照组、CLP组和CLP + EA组。术后连续3天在双侧ST36进行EA。术后第7天观察各组7天生存率。术后第8 ~ 12天采用Morris水迷宫(MWM)测试认知功能。采用透射电镜(TEM)和转移酶dUTP镍端标记(TUNEL)染色分别测定海马神经元膜的结构完整性和海马组织中存活神经元的数量。采用Western blotting和实时荧光定量聚合酶链反应(RT-qPCR)检测海马CA1神经元中核苷酸结合结构域样受体蛋白1 (NLRP1)、caspase-1和gasdermin-D (GSDM D)的表达，采用酶联免疫吸附法检测caspase-1浓度。结果:与CLP组比较，CLP + EA组7天生存率及认知功能均有显著提高。EA处理后，海马CA1神经元膜完整性和海马神经元死亡率显著降低，NLRP1、caspase-1和GSDM D表达下调。结论:EA可减轻脓毒症小鼠的认知功能障碍和神经元焦亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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