LINC00891 Attenuates the Proliferation and Metastasis of Osteosarcoma Cells via miR-27a-3p/TET1 Axis.

IF 1.1 4区 生物学 Q4 GENETICS & HEREDITY Genetic testing and molecular biomarkers Pub Date : 2023-08-01 DOI:10.1089/gtmb.2023.0163
Shufang Zhang, Rongchun Chen
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Abstract

Objective: There is currently no adequate treatment for osteosarcoma, a bone malignancy that poses a serious threat to adolescents and children. The dysregulation of long noncoding RNAs is associated with many cancers, including osteosarcoma. LINC00891 expression is aberrant in endometrial cancer, lung cancer, and thyroid cancer, and likely regulate the malignant behavior of cancer. However, the potential function and mechanisms of LINC00891 in osteosarcoma progression remain unclear. Materials and Methods: LINC00891, miR-27a-3p, and TET1 mRNA expression in osteosarcoma cells were analyzed using quantitative reverse transcription-polymerase chain reaction. CCK-8 and Transwell experiments were performed on osteosarcoma cells to investigate proliferation, migration, and invasion, respectively. Ten-eleven translocation 1 (TET1) protein was analyzed using western blotting. Luciferase experiment was performed to investigate the interactions between LINC00891 with miR-27a-3p, and miR-27a-3p with TET1. Results: LINC00891 expression was dramatically decreased in the five osteosarcoma cell lines examined, particularly in 143B and SaoS-2 cells. LINC00891 overexpression due to plasmid transfection sharply blocked the proliferation, migration, and invasion of osteosarcoma cells. Dual-luciferase reporter experiments found that LINC00891 sponges miR-27a-3p, and LINC00891 overexpression sharply decreases miR-27a-3p expression. Transfection with miR-27a-3p mimic accelerated the malignant behaviors in LINC00891 overexpressed-osteosarcoma cells. Moreover, TET1 was a novel targeted-gene of miR-27a-3p. TET1 protein was significantly impeded, whereas LINC00891 overexpression elevated TET1 mRNA and protein in osteosarcoma cells. MiR-27a-3p overexpression inhibited TET1 mRNA and protein in osteosarcoma cells. Conclusions: Our study verified that LINC00891 attenuates the proliferation and metastasis of osteosarcoma cells via the miR-27a-3p/TET1 axis. This study clarifies a new mechanism and therapeutic target for the development of osteosarcoma.

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LINC00891通过miR-27a-3p/TET1轴减弱骨肉瘤细胞的增殖和转移。
目的:骨肉瘤是一种严重威胁青少年和儿童的骨恶性肿瘤,目前尚无足够的治疗方法。长链非编码rna的失调与许多癌症有关,包括骨肉瘤。LINC00891在子宫内膜癌、肺癌和甲状腺癌中表达异常,可能调控肿瘤的恶性行为。然而,LINC00891在骨肉瘤进展中的潜在功能和机制尚不清楚。材料与方法:采用定量逆转录-聚合酶链反应分析骨肉瘤细胞中LINC00891、miR-27a-3p和TET1 mRNA的表达。CCK-8和Transwell实验分别对骨肉瘤细胞进行增殖、迁移和侵袭实验。western blotting检测10 - 11易位1 (TET1)蛋白。我们通过荧光素酶实验来研究LINC00891与miR-27a-3p以及miR-27a-3p与TET1的相互作用。结果:LINC00891在5种骨肉瘤细胞系中表达显著降低,尤其是在143B和SaoS-2细胞中。质粒转染后LINC00891过表达可明显阻断骨肉瘤细胞的增殖、迁移和侵袭。双荧光素酶报告基因实验发现,LINC00891抑制miR-27a-3p,过表达LINC00891可显著降低miR-27a-3p的表达。转染miR-27a-3p模拟物加速了LINC00891过表达骨肉瘤细胞的恶性行为。此外,TET1是miR-27a-3p的一个新的靶向基因。骨肉瘤细胞中TET1蛋白表达明显受阻,而LINC00891过表达升高TET1 mRNA和蛋白表达。MiR-27a-3p过表达抑制骨肉瘤细胞TET1 mRNA和蛋白表达。结论:我们的研究证实了LINC00891通过miR-27a-3p/TET1轴减弱骨肉瘤细胞的增殖和转移。本研究为骨肉瘤的发生提供了新的机制和治疗靶点。
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来源期刊
CiteScore
2.50
自引率
7.10%
发文量
63
审稿时长
1 months
期刊介绍: Genetic Testing and Molecular Biomarkers is the leading peer-reviewed journal covering all aspects of human genetic testing including molecular biomarkers. The Journal provides a forum for the development of new technology; the application of testing to decision making in an increasingly varied set of clinical situations; ethical, legal, social, and economic aspects of genetic testing; and issues concerning effective genetic counseling. This is the definitive resource for researchers, clinicians, and scientists who develop, perform, and interpret genetic tests and their results. Genetic Testing and Molecular Biomarkers coverage includes: -Diagnosis across the life span- Risk assessment- Carrier detection in individuals, couples, and populations- Novel methods and new instrumentation for genetic testing- Results of molecular, biochemical, and cytogenetic testing- Genetic counseling
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