Methodological approach: using a within-subjects design in the marble burying assay.

IF 1.6 4区 心理学 Q3 BEHAVIORAL SCIENCES Behavioural Pharmacology Pub Date : 2023-12-01 Epub Date: 2023-08-31 DOI:10.1097/FBP.0000000000000752
Kaitlyn J Partridge, Ruth Olson, Todd M Hillhouse
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Abstract

In 2016, the National Institutes of Health mandated the use of both male and female mice in funded research. The use of both sexes is an important variable to consider; however, it comes with negative consequences such as increased animal expenses. One way to combat these negatives is to explore the option of using a within-subjects design (repeated measures) in behavioral assays that historically use a between-subjects design. Our study aimed to determine if a within-subjects design can be utilized in the marble burying assay. The marble burying assay is used as a tool for screening putative anxiolytic compounds as the assay is thought to measure obsessive-compulsive disorder- or anxiety-like behaviors. First, we compared the effects of sex and digging medium (corn cob or Sani Chip) on the number of marbles buried using CD-1 mice. Second, we determined if mice would continue to bury marbles after repeated exposures to the test arena. Lastly, we tested three positive controls (buspirone, ketamine, and fluoxetine). We found that mice buried significantly more marbles within Sani Chip digging medium, and no sex differences were observed. Next, the number of marbles buried and locomotor activity remained consistent across four test sessions. The positive controls buspirone (3.2-10 mg/kg) ketamine (32 mg/kg), and fluoxetine (10 mg/kg) decreased the number of marbles buried using the within-subjects design. These data suggest that a within-subjects design is optimal for the marble burying assay as it will reduce the number of animals and increase statistical power.

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方法论方法:在大理石掩埋试验中使用受试者内部设计。
2016年,美国国立卫生研究院强制要求在资助的研究中同时使用雄性和雌性小鼠。两性的使用是一个需要考虑的重要变量;然而,它也带来了负面后果,比如动物费用的增加。对抗这些负面影响的一种方法是探索在行为分析中使用受试者内部设计(重复测量)的选项,该方法历史上使用受试对象之间的设计。我们的研究旨在确定受试者内部设计是否可以用于大理石掩埋试验。大理石掩埋法被用作筛选公认的抗焦虑化合物的工具,因为该法被认为可以测量强迫症或类似焦虑的行为。首先,我们比较了性别和挖掘介质(玉米芯或Sani Chip)对CD-1小鼠埋葬弹珠数量的影响。其次,我们确定了老鼠在反复暴露于试验场后是否会继续埋葬弹珠。最后,我们测试了三种阳性对照(丁螺环酮、氯胺酮和氟西汀)。我们发现,小鼠在Sani Chip挖掘介质中埋下的弹珠明显更多,并且没有观察到性别差异。接下来,埋在地下的弹珠数量和运动活动在四次测试中保持一致。阳性对照丁螺环酮(3.2-10 mg/kg)氯胺酮(32 mg/kg)和氟西汀(10 mg/kg)降低了使用受试者内部设计掩埋的弹珠的数量。这些数据表明,受试者内部设计是大理石埋葬试验的最佳设计,因为它将减少动物数量并提高统计能力。
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来源期刊
Behavioural Pharmacology
Behavioural Pharmacology 医学-行为科学
CiteScore
3.40
自引率
0.00%
发文量
84
审稿时长
6-12 weeks
期刊介绍: Behavioural Pharmacology accepts original full and short research reports in diverse areas ranging from ethopharmacology to the pharmacology of schedule-controlled operant behaviour, provided that their primary focus is behavioural. Suitable topics include drug, chemical and hormonal effects on behaviour, the neurochemical mechanisms under-lying behaviour, and behavioural methods for the study of drug action. Both animal and human studies are welcome; however, studies reporting neurochemical data should have a predominantly behavioural focus, and human studies should not consist exclusively of clinical trials or case reports. Preference is given to studies that demonstrate and develop the potential of behavioural methods, and to papers reporting findings of direct relevance to clinical problems. Papers making a significant theoretical contribution are particularly welcome and, where possible and merited, space is made available for authors to explore fully the theoretical implications of their findings. Reviews of an area of the literature or at an appropriate stage in the development of an author’s own work are welcome. Commentaries in areas of current interest are also considered for publication, as are Reviews and Commentaries in areas outside behavioural pharmacology, but of importance and interest to behavioural pharmacologists. Behavioural Pharmacology publishes frequent Special Issues on current hot topics. The editors welcome correspondence about whether a paper in preparation might be suitable for inclusion in a Special Issue.
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