Luteolin photo-protects zebrafish from environmental stressor ultraviolet radiation (UVB).

IF 2.3 4区 医学 Q3 ENVIRONMENTAL SCIENCES Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2023-10-02 Epub Date: 2023-08-23 DOI:10.1080/15287394.2023.2249944
Gokul Sudhakaran, Madesh Selvam, A R Sreekutty, Abhirami Chandran, Bader O Almutairi, Selvaraj Arokiyaraj, Pachaiappan Raman, Ajay Guru, Jesu Arockiaraj
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引用次数: 1

Abstract

Ultraviolet B wavelength ray radiation (UVB) is an environmental stressor with detrimental effects to the aquatic and human systems but also enhances adverse effects when combined with several other environmental factors such as temperature and pollution. UV rays induce cellular oxidative damage and impair motility. This study aimed to examine the photo-protective activity of flavonoid luteolin against UV-B irradiation-induced oxidative stress and cellular damage using zebrafish. An in-vivo photoaging model was established using UV-B irradiation in zebrafish larvae exposed to 100 mJ/cm2. Data demonstrated that UV-B irradiation of swimming water enhanced production of ROS and superoxide anions as well as depleted total glutathione levels in zebrafish larvae. UV-B irradiation also triggered cellular damage and membrane rupture in zebra fish. Further, 100 mJ/cm2 of UV-B radiation exposure to adult-wild type zebrafish co-exposed with intraperitoneally (ip) injected luteolin upregulated the local neuroendocrine axes by activating vascular endothelial growth factor (VEGF) and elevating levels of pro-inflammatory cytokines IL-1β and TNF-α. Histologically, UV-B irradiation induced skin lesions and locomotory defects with clumping and degeneration of brain glial cells. However, luteolin effectively inhibited the excess production of reactive oxygen species (ROS) and decreased superoxide anion levels induced by UV-B irradiation. Luteolin restored the depleted glutathione levels. In addition, luteolin blocked apoptosis and lipidperoxidation. Luteolin protected adult zebrafish by downregulating the pro-inflammatory cytokine protein expression levels and diminishing VEGF activation. Luteolin also alleviated locomotory defects by inhibiting activation of microglia and inflammatory responses by preventing accumulation of glial cells and vacuolation. Data demonstrate that luteolin may protect zebrafish from UV-B-induced photodamage through DNA-protective, antioxidant and anti-inflammatory responses.

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木犀草素照片保护斑马鱼免受环境压力紫外线辐射(UVB)的影响。
紫外线B波长射线辐射(UVB)是一种环境压力源,对水生和人类系统具有有害影响,但与温度和污染等其他几种环境因素相结合时,也会增强不利影响。紫外线会引起细胞氧化损伤并损害运动能力。本研究旨在检测黄酮木犀草素对UV-B辐射诱导的氧化应激和斑马鱼细胞损伤的光保护活性。使用UV-B辐射在暴露于100mJ/cm2的斑马鱼幼虫中建立体内光老化模型。数据表明,游泳水中的UV-B辐射增强了斑马鱼幼虫中ROS和超氧化物阴离子的产生,并降低了总谷胱甘肽的水平。UV-B辐射也引发斑马鱼细胞损伤和膜破裂。此外,100 mJ/cm2的UV-B辐射暴露于与腹膜内(ip)注射木犀草素共同暴露的成年野生型斑马鱼,通过激活血管内皮生长因子(VEGF)和升高促炎细胞因子IL-1β和TNF-α的水平来上调局部神经内分泌轴。组织学上,UV-B辐射诱导皮肤损伤和运动缺陷,伴有脑胶质细胞的聚集和变性。然而,木犀草素有效地抑制了UV-B辐射诱导的活性氧(ROS)的过量产生,并降低了超氧化物阴离子水平。木犀草素恢复了耗竭的谷胱甘肽水平。此外,木犀草素阻断细胞凋亡和脂质过氧化。木犀草素通过下调促炎细胞因子蛋白表达水平和减少VEGF激活来保护成年斑马鱼。木犀草素还通过抑制小胶质细胞的活化和通过防止神经胶质细胞的积聚和空泡化来减轻运动缺陷。数据表明,木犀草素可以通过DNA保护、抗氧化和抗炎反应保护斑马鱼免受UV-B诱导的光损伤。
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来源期刊
CiteScore
5.20
自引率
19.20%
发文量
46
审稿时长
8-16 weeks
期刊介绍: The Journal of Toxicology and Environmental Health, Part A , Current Issues is an authoritative journal that features strictly refereed original research in the field of environmental sciences, public and occupational health, and toxicology.
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