Thymol-Loaded Polymeric Nanoparticles Improve the Postharvest Microbiological Safety of Blueberries.

IF 2.3 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Food Technology and Biotechnology Pub Date : 2023-06-01 DOI:10.17113/ftb.61.02.23.7595
Syarifah Ab Rashid, Woei-Yenn Tong, Chean-Ring Leong, Wen-Nee Tan, Chee-Keong Lee, Mohd Razealy Anuar, Siew-Hway Teo, Siti Khalida Abdull Lazit, Jun-Wei Lim, Nur Amiera Syuhada Rozman
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Abstract

Research background: The presence of Yersinia enterocolitica on raw food products raises the concern of yersiniosis as most of the berries are consumed raw. This is a challenging issue from the food safety aspect since it could increase the occurrence of foodborne diseases among humans. Thus, it is crucial to implement an effective sanitation before the packaging.

Experimental approach: This study aims to synthesize and characterize thymol-loaded polyvinyl alcohol (Thy/PVA) nanoparticles as a sanitizer for postharvest treatment of blueberries. Thy/PVA nanoparticles were characterized by spectroscopic and microscopic approaches, prior to the analyses of antimicrobial properties.

Results and conclusions: The diameter size of the nanoparticles was on average 84.7 nm, with a surface charge of -11.73 mV. Based on Fourier transform infrared (FTIR) measurement, the Thy/PVA nanoparticles notably shifted to the frequency of 3275.70, 2869.66, 1651.02 and 1090.52 cm-1. A rapid burst was observed in the first hour of release study, and 74.9 % thymol was released from the PVA nanoparticles. The largest inhibition zone was displayed by methicillin-resistant Staphylococcus aureus (MRSA), followed by Y. enterocolitica and Salmonella typhi. However, amongst these bacteria, the inhibition and killing of Y. enterocolitica required a lower concentration of Thy/PVA nanoparticles. The treatment successfully reduced the bacterial load of Y. enterocolitica on blueberries by 100 %.

Novelty and scientific contribution: Thymol is a plant-based chemical without reported adverse effects to humans. In this study, by using the nanotechnology method of encapsulation with PVA, we improved the stability and physicochemical properties of thymol. This nanoparticle-based sanitizer could potentially promote the postharvest microbiological safety of raw berries, which may become an alternative practice of food safety.

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载胸腺酚聚合物纳米颗粒提高蓝莓采后微生物安全性。
研究背景:生食产品中存在小肠结肠炎耶尔森菌引起了人们对耶尔森菌病的关注,因为大多数浆果都是生食的。从食品安全的角度来看,这是一个具有挑战性的问题,因为它可能增加人类食源性疾病的发生。因此,在包装前实施有效的卫生是至关重要的。实验方法:本研究旨在合成并表征装载百里香的聚乙烯醇(Thy/PVA)纳米颗粒作为蓝莓采后处理的消毒剂。在抗菌性能分析之前,通过光谱和显微镜方法对Thy/PVA纳米颗粒进行了表征。结果与结论:纳米颗粒的平均直径为84.7 nm,表面电荷为-11.73 mV。基于傅里叶变换红外(FTIR)测量,Thy/PVA纳米颗粒明显向3275.70、2869.66、1651.02和1090.52 cm-1的频率偏移。在释放研究的第一个小时内观察到快速爆发,74.9%的百里香酚从PVA纳米颗粒中释放出来。耐甲氧西林金黄色葡萄球菌(MRSA)的抑制区最大,其次是小肠结肠炎耶氏菌和伤寒沙门氏菌。然而,在这些细菌中,抑制和杀死小肠结肠炎Y.需要较低浓度的Thy/PVA纳米颗粒。该处理成功地将蓝莓上小肠结肠炎耶氏菌的细菌负荷降低了100%。新颖性和科学贡献:百里香酚是一种基于植物的化学物质,对人体没有不良影响。本研究采用聚乙烯醇包封的纳米技术,提高了百里香酚的稳定性和理化性质。这种基于纳米粒子的消毒剂有可能促进生浆果采后微生物的安全性,这可能成为食品安全的一种替代做法。
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来源期刊
Food Technology and Biotechnology
Food Technology and Biotechnology 工程技术-生物工程与应用微生物
CiteScore
3.70
自引率
0.00%
发文量
33
审稿时长
12 months
期刊介绍: Food Technology and Biotechnology (FTB) is a diamond open access, peer-reviewed international quarterly scientific journal that publishes papers covering a wide range of topics, including molecular biology, genetic engineering, biochemistry, microbiology, biochemical engineering and biotechnological processing, food science, analysis of food ingredients and final products, food processing and technology, oenology and waste treatment. The Journal is published by the University of Zagreb, Faculty of Food Technology and Biotechnology, Croatia. It is an official journal of Croatian Society of Biotechnology and Slovenian Microbiological Society, financed by the Croatian Ministry of Science and Education, and supported by the Croatian Academy of Sciences and Arts.
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