Aging: silencing the PKA and AkT/PKB signaling pathways alters the antioxidant capacity of resveratrol.

IF 4.4 4区 医学 Q1 GERIATRICS & GERONTOLOGY Biogerontology Pub Date : 2023-12-01 Epub Date: 2023-07-17 DOI:10.1007/s10522-023-10046-x
Filipe Nogueira Franco, Luciana de Cassia Cardoso, Bárbara Néllita Moura Silva, Glaucy Rodrigues de Araújo, Miriam Martins Chaves
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Abstract

One of the theories related to aging is the increase in oxidative stress. Given this, the objective of the study is to evaluate the cellular mechanisms responsible for the resveratrol antioxidant effect on leukocytes from donors aged between 20 and 80 years old. For this, leukocytes from donors of three age groups (20-39, 40-59 and 60-80) were isolated. Image-iT™LIVE Green Reactive Oxygen Species (ROS) Kit was used. Reactive Nitrogen Species (RNS) analysis was performed by measuring nitric oxide and peroxynitrite. The PKA, Akt/PKB and p38-MAPK were evaluated by chemiluminescence. The statistical analysis between age and treatments were performed by Pearson correlation (*p < 0.05). It was possible to observe the antioxidant effect of resveratrol in all age groups. The correlation results show loss of resveratrol effect in decreasing ROS in leukocytes from older donors. We observed an active antioxidant effect of p38-MAPK in all ages, with resveratrol acting on it. The PKA and Akt/PKB were active in leukocytes from donors aged 20-59. In cells from donors older than 60, these pathways are silenced, and an effect is also not observed in cells treated with resveratrol. Therefore, resveratrol showed antioxidant effect in all age, although it was more pronounced in leukocytes from younger. One of resveratrol's mechanisms is due to the activation of the PKA and Akt/PKB, which were activated in younger donor cells.

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衰老:使PKA和AkT/PKB信号通路沉默会改变白藜芦醇的抗氧化能力。
与衰老有关的理论之一是氧化应激的增加。鉴于此,本研究的目的是评估白藜芦醇对20至80岁供体白细胞抗氧化作用的细胞机制。为此,从三个年龄组(20-39、40-59和60-80)的捐献者中分离出白细胞。图像iT™使用活性氧(ROS)试剂盒。通过测量一氧化氮和过氧亚硝酸盐进行活性氮物种(RNS)分析。用化学发光法测定PKA、Akt/PKB和p38MAPK。年龄和治疗之间的统计分析采用Pearson相关(*p
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来源期刊
Biogerontology
Biogerontology 医学-老年医学
CiteScore
8.00
自引率
4.40%
发文量
54
审稿时长
>12 weeks
期刊介绍: The journal Biogerontology offers a platform for research which aims primarily at achieving healthy old age accompanied by improved longevity. The focus is on efforts to understand, prevent, cure or minimize age-related impairments. Biogerontology provides a peer-reviewed forum for publishing original research data, new ideas and discussions on modulating the aging process by physical, chemical and biological means, including transgenic and knockout organisms; cell culture systems to develop new approaches and health care products for maintaining or recovering the lost biochemical functions; immunology, autoimmunity and infection in aging; vertebrates, invertebrates, micro-organisms and plants for experimental studies on genetic determinants of aging and longevity; biodemography and theoretical models linking aging and survival kinetics.
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