Whole Genome Sequencing of an Extensively Drug-Resistant Raoultella planticola Isolate Containing bla_KPC-2, bla_NDM-1, and bla_CTX-M-15.

IF 2.3 4区医学 Q3 INFECTIOUS DISEASES Microbial drug resistance Pub Date : 2023-09-01 DOI:10.1089/mdr.2022.0229

Paulo Victor Batista Marini, Eliandro Reis Tavares, Cintia Werner Motter, Letícia Busato Migliorini, Romário Oliveira de Sales, Nayara Helisandra Fedrigo, Danielle Rosani Shinohara, Mariangela Hungria, Sueli Fumie Yamada-Ogatta, Maria Cristina Bronharo Tognim

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Abstract

Raoultella planticola harboring genes that confer resistance to antimicrobials, such as carbapenems, have been associated with severe infections in immunocompromised patients. In this study, we reported the first whole genome sequence of a Brazilian isolate of R. planticola and the genomic context of antibiotic resistance markers. By whole-genome sequencing (WGS) of a carbapenem-resistant R. planticola isolate, RpHUM1, we found 23 resistance-encoding genes belonging to 9 classes of antibiotics (aminoglycosides, β-lactams, fluoroquinolones, fosfomycin, macrolides, phenicols, sulfonamides, tetracycline, and diaminopyrimidine derivatives) and 3 plasmids (RpHUM1pEaer-4382s, RpHUM1_pFDAARGOS_440, and RpHUM1pRSF1010). This isolate coharbored the genes bla_KPC-2, which is carried by the plasmid RpHUM1pEaer-4382s, and bla_NDM-1 and bla_CTX-M-15 all located in the accessory genome. In addition, these genes were associated with, at least, one mobile genetic element. This comprehensive knowledge is of great importance for implementation of control measures to prevent the rapid dissemination of this neglected microorganism and their genetic resistance background.

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含有blaKPC-2、blaNDM-1和blaCTX-M-15的广泛耐药的planticola Raoultella分离物全基因组测序

含有抗微生物药物(如碳青霉烯类)耐药性基因的车前拉乌尔tella planticola与免疫功能低下患者的严重感染有关。在这项研究中，我们报道了第一个巴西分离菌的全基因组序列和抗生素耐药性标记的基因组背景。通过对一株碳青霉烯类耐药菌株RpHUM1的全基因组测序(WGS)，我们发现了23个耐药编码基因，分别属于9类抗生素(氨基糖苷类、β-内酰胺类、氟喹诺酮类、磷霉素类、大环内酯类、酚类、磺胺类、四环素类和二氨基嘧啶类衍生物)和3个质粒(rphum1peaer4382s、RpHUM1_pFDAARGOS_440和RpHUM1pRSF1010)。该分离物含有blaKPC-2基因，该基因由质粒rphum1peer -4382携带，blaNDM-1和blaCTX-M-15均位于辅助基因组中。此外，这些基因至少与一种可移动遗传元件相关。这种全面的知识对于实施控制措施以防止这种被忽视的微生物及其遗传抗性背景的快速传播具有重要意义。

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来源期刊

Microbial drug resistance 医学-传染病学

CiteScore

6.00

自引率

3.80%

发文量

118

审稿时长

6-12 weeks

期刊介绍： Microbial Drug Resistance (MDR) is an international, peer-reviewed journal that covers the global spread and threat of multi-drug resistant clones of major pathogens that are widely documented in hospitals and the scientific community. The Journal addresses the serious challenges of trying to decipher the molecular mechanisms of drug resistance. MDR provides a multidisciplinary forum for peer-reviewed original publications as well as topical reviews and special reports. MDR coverage includes: Molecular biology of resistance mechanisms Virulence genes and disease Molecular epidemiology Drug design Infection control.