Ecdysone-induced microRNA miR-276a-3p controls developmental growth by targeting the insulin-like receptor in Drosophila

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Insect Molecular Biology Pub Date : 2023-09-13 DOI:10.1111/imb.12872
Sojeong Lee, Nayeon Kim, Daegyu Jang, Hee Kyung Kim, Jongin Kim, Ji Won Jeon, Do-Hwan Lim
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Abstract

Animal growth is controlled by a variety of external and internal factors during development. The steroid hormone ecdysone plays a critical role in insect development by regulating the expression of various genes. In this study, we found that fat body-specific expression of miR-276a, an ecdysone-responsive microRNA (miRNA), led to a decrease in the total mass of the larval fat body, resulting in significant growth reduction in Drosophila. Changes in miR-276a expression also affected the proliferation of Drosophila S2 cells. Furthermore, we found that the insulin-like receptor (InR) is a biologically relevant target gene regulated by miR-276a-3p. In addition, we found that miR-276a-3p is upregulated by the canonical ecdysone signalling pathway involving the ecdysone receptor and broad complex. A reduction in cell proliferation caused by ecdysone was compromised by blocking miR-276a-3p activity. Thus, our results suggest that miR-276a-3p is involved in ecdysone-mediated growth reduction by controlling InR expression in the insulin signalling pathway.

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脱皮激素诱导的microRNA miR-276a-3p通过靶向果蝇的胰岛素样受体来控制发育生长。
动物的生长在发育过程中受到各种外部和内部因素的控制。类固醇激素蜕皮激素通过调节各种基因的表达在昆虫发育中起着关键作用。在这项研究中,我们发现脂肪体特异性表达miR-276a,一种蜕皮激素反应性微小RNA(miRNA),导致幼虫脂肪体的总质量减少,导致果蝇的生长显著减少。miR-276a表达的变化也影响果蝇S2细胞的增殖。此外,我们发现胰岛素样受体(InR)是由miR-276a-3p调节的生物学相关靶基因。此外,我们发现miR-276a-3p通过涉及蜕皮激素受体和广泛复合物的典型蜕皮激素信号通路上调。蜕皮激素引起的细胞增殖减少通过阻断miR-276a-3p活性而受到损害。因此,我们的研究结果表明,miR-276a-3p通过控制胰岛素信号通路中的InR表达,参与蜕皮激素介导的生长减少。
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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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