Plasma MicroRNAs Related to Metabolic Syndrome in Mexican Women.

IF 2 4区 医学 Q3 GENETICS & HEREDITY Lifestyle Genomics Pub Date : 2023-01-01 Epub Date: 2023-09-14 DOI:10.1159/000534041
Marisol Adelina Ramírez-Solano, Emilio J Córdova, Lorena Orozco, María Elizabeth Tejero
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Abstract

Introduction: The metabolic syndrome (MetS) is a cluster of abnormalities related to cardiovascular disease (CVD). Circulating miRNAs (c-miRNAs) are non-coding RNAs associated with different phenotypes, some of them integrating the MetS. The aim of the study was to compare the c-miRNAs profile in plasma between women with MetS and controls and explore their possible association with dysregulation of metabolic pathways.

Methods: The study was conducted in two phases. At the screening phase, miRNA composition in fasting plasma was compared between 8 participants with MetS and 10 healthy controls, using microarray technology. The validation phase included the analysis by qRT-PCR of 10 selected c-miRNAs in an independent sample (n = 29).

Results: We found 21 c-miRNAs differentially expressed between cases and controls. The concentration in plasma of the c-miRNAs hsa-miR-1260a, hsa-miR-4514, and hsa-miR-4687-5p were also correlated with risk factors for CVD. Differences of hsa-miR-1260a between cases and controls were validated using qRT-PCR (fold-change = 7.0; p = 0.003).

Conclusion: The signature of plasma c-miRNAs differed between women with MetS and controls. The identified miRNAs regulate pathways related to the MetS such as insulin resistance and adipokine activity. The role of c-miR-1260a in the MetS remains to be elucidated.

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墨西哥妇女血浆中与代谢综合征相关的微RNA。
简介代谢综合征(MetS)是一组与心血管疾病(CVD)相关的异常现象。循环 miRNAs(c-miRNAs)是与不同表型相关的非编码 RNAs,其中一些与 MetS 相关。本研究旨在比较 MetS 妇女和对照组血浆中的 c-miRNAs 谱,并探讨它们与代谢途径失调的可能关联:研究分两个阶段进行。在筛选阶段,利用芯片技术比较了 8 名 MetS 患者和 10 名健康对照者空腹血浆中的 miRNA 组成。验证阶段包括通过 qRT-PCR 对独立样本(n = 29)中 10 个选定的 c-miRNA 进行分析:结果:我们发现 21 个 c-miRNA 在病例和对照组之间有差异表达。血浆中 c-miRNA hsa-miR-1260a、hsa-miR-4514 和 hsa-miR-4687-5p 的浓度也与心血管疾病的危险因素相关。通过 qRT-PCR 验证了病例与对照组之间 hsa-miR-1260a 的差异(折变 = 7.0;p = 0.003):结论:患有 MetS 的女性与对照组之间血浆 c-miRNA 的特征存在差异。结论:血浆中的 c-miRNAs 特征在 MetS 妇女和对照组之间存在差异。所发现的 miRNAs 可调控与 MetS 相关的通路,如胰岛素抵抗和脂肪因子活性。c-miR-1260a 在 MetS 中的作用仍有待阐明。
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来源期刊
Lifestyle Genomics
Lifestyle Genomics Agricultural and Biological Sciences-Food Science
CiteScore
4.00
自引率
7.70%
发文量
11
审稿时长
28 weeks
期刊介绍: Lifestyle Genomics aims to provide a forum for highlighting new advances in the broad area of lifestyle-gene interactions and their influence on health and disease. The journal welcomes novel contributions that investigate how genetics may influence a person’s response to lifestyle factors, such as diet and nutrition, natural health products, physical activity, and sleep, amongst others. Additionally, contributions examining how lifestyle factors influence the expression/abundance of genes, proteins and metabolites in cell and animal models as well as in humans are also of interest. The journal will publish high-quality original research papers, brief research communications, reviews outlining timely advances in the field, and brief research methods pertaining to lifestyle genomics. It will also include a unique section under the heading “Market Place” presenting articles of companies active in the area of lifestyle genomics. Research articles will undergo rigorous scientific as well as statistical/bioinformatic review to ensure excellence.
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