FGF21 promotes angiotensin II-induced abdominal aortic aneurysm via PI3K/AKT/mTOR pathway.

IF 1 4区医学 Q4 PERIPHERAL VASCULAR DISEASE Vascular Pub Date : 2024-12-01 Epub Date: 2023-07-31 DOI:10.1177/17085381231192688

Xuefeng Gu, Qi Li, Tianwei Qian, Qi Hu, Jianfeng Gu, Wei Ding, Ming Li, Ming Wang, Huan Lu, Ke Tao

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Abstract

Background: Abdominal aortic aneurysm (AAA) is a potentially fatal vascular disorder with a high mortality rate. It was previously reported that fibroblast growth factor 21 (FGF21) was highly expressed in AAA patients. Nonetheless, its underlying mechanism in AAA progression is unclarified.

Methods: Angiotensin II (Ang-II) was used to induce AAA in human aortic vascular smooth muscle cells (HASMCs) and mouse models. Western blotting and RT-qPCR were utilized for measuring protein and RNA levels. Immunofluorescence staining was utilized for detecting LC3B expression in HASMCs. Elastica van Gieson staining was conducted for histological analysis of the abdominal aortas of mice.

Results: FGF21 displayed a high level in Ang-II-stimulated HASMCs and AAA mice. FGF21 depletion ameliorated abdominal aorta dilation and Ang-II-triggered pathological changes in mice. FGF21 silencing hindered autophagy and PI3K/AKT/mTOR pathway.

Conclusions: FGF21 contributes to AAA progression by enhancing autophagy and activating PI3K/AKT/mTOR pathway.

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FGF21通过PI3K/AKT/mTOR途径促进血管紧张素II诱发的腹主动脉瘤。

背景：腹主动脉瘤（AAA腹主动脉瘤（AAA）是一种潜在的致命性血管疾病，死亡率很高。此前有报道称，成纤维细胞生长因子 21（FGF21）在 AAA 患者中高表达。然而，FGF21在AAA进展中的潜在机制尚未明确：方法：使用血管紧张素 II（Ang-II）诱导人主动脉血管平滑肌细胞（HASMCs）和小鼠模型中的 AAA。方法：利用血管紧张素 II（Ang-II）诱导人主动脉血管平滑肌细胞（HASMCs）和小鼠模型中的 AAA。免疫荧光染色用于检测 HASMCs 中 LC3B 的表达。用 Elastica van Gieson 染色法对小鼠腹主动脉进行组织学分析：结果：FGF21在Ang-II刺激的HASMCs和AAA小鼠中显示出较高水平。结果：FGF21 在 Ang-II 刺激的 HASMCs 和 AAA 小鼠中显示出较高的水平。FGF21沉默阻碍了自噬和PI3K/AKT/mTOR通路：结论：FGF21通过增强自噬和激活PI3K/AKT/mTOR通路促进AAA的进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Vascular 医学-外周血管病

CiteScore

2.30

自引率

9.10%

发文量

196

审稿时长

6-12 weeks

期刊介绍： Vascular provides readers with new and unusual up-to-date articles and case reports focusing on vascular and endovascular topics. It is a highly international forum for the discussion and debate of all aspects of this distinct surgical specialty. It also features opinion pieces, literature reviews and controversial issues presented from various points of view.