Differences of Escherichia coli isolated from different organs of the individual sheep: molecular typing, antibiotics resistance, and biofilm formation.

IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Folia microbiologica Pub Date : 2024-06-01 Epub Date: 2023-08-04 DOI:10.1007/s12223-023-01082-8
Zihao Wu, Haoming Chi, Tingting Han, Guangxi Li, Jixue Wang, Wei Chen
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Abstract

Despite numerous studies on Escherichia coli (E. coli) from sheep, there have been few reports on the characterization of E. coli isolates from various organs of individual sheep until now. The present study conducted molecular typing, antibiotics resistance, biofilm formation, and virulence genes on E. coli isolated from 57 freshly slaughtered apparently healthy sheep carcasses, gallbladders, fecal samples, and mesenteric lymph nodes (MLNs). The results demonstrated that the detection rate of R1 LPS core type in E. coli isolated from fecal samples (70.83%) was higher than that from other organs, but the detection rate of antibiotic resistance genes was lower (P < 0.05). The predominant phylogenetic group of E. coli isolated from the carcasses was group B1 (93.33%), and the detection rate of multidrug-resistance phenotype (80%) and the resistance rate of E. coli was higher than that from other organs (P < 0.05). Interestingly, the intensity of biofilm formation of E. coli isolated from MLNs was higher than that from other organs (P < 0.05). However, except for ibeB, the detection rates of virulence genes did not differ in E.coli isolated from different organs. In conclusion, differences were noted in these parameters of E. coli isolated from different organs of individual sheep. Therefore, the data may contain considerable mistakes concerning the actual situation in the host if we only analyze the data of E. coli isolated from feces or carcasses.

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从绵羊不同器官中分离出的大肠埃希菌的差异:分子分型、抗生素耐药性和生物膜形成。
尽管对绵羊大肠埃希氏菌(E. coli)进行了大量研究,但迄今为止,关于从绵羊各个器官分离出的大肠埃希氏菌特征的报道却寥寥无几。本研究对从 57 只刚宰杀的明显健康的绵羊尸体、胆囊、粪便样本和肠系膜淋巴结(MLN)中分离出的大肠杆菌进行了分子分型、抗生素耐药性、生物膜形成和毒力基因分析。结果表明,从粪便样本中分离的大肠杆菌的 R1 LPS 核心型检出率(70.83%)高于从其他器官中分离的大肠杆菌,但抗生素耐药基因的检出率较低(P<0.05)。
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来源期刊
Folia microbiologica
Folia microbiologica 工程技术-生物工程与应用微生物
CiteScore
5.80
自引率
0.00%
发文量
82
审稿时长
6-12 weeks
期刊介绍: Unlike journals which specialize ever more narrowly, Folia Microbiologica (FM) takes an open approach that spans general, soil, medical and industrial microbiology, plus some branches of immunology. This English-language journal publishes original papers, reviews and mini-reviews, short communications and book reviews. The coverage includes cutting-edge methods and promising new topics, as well as studies using established methods that exhibit promise in practical applications such as medicine, animal husbandry and more. The coverage of FM is expanding beyond Central and Eastern Europe, with a growing proportion of its contents contributed by international authors.
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