A facile electrochemical biosensor for coronavirus RNA assay with silver deposition.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL Talanta Pub Date : 2024-01-01 Epub Date: 2023-07-30 DOI:10.1016/j.talanta.2023.125013

Yaqi Hu, Fang Bao, Siyuan Fu, Shiyuan Feng, Jinfeng Miao, Peng Miao, Yuanyuan Xu

引用次数: 0

Abstract

Coronaviruses are highly infectious and pose a serious threat to human and animal healths. In this work, a facile electrochemical method based on Exonuclease III (Exo III) catalyzed digestion and silver deposition is developed for coronavirus RNA analysis. A magnetic separation procedure is performed to specifically identify target sequence and release single-stranded DNA modified gold nanoparticles (AuNPs). The nanoparticles can thus be immobilized at a screen-printed electrode and catalyze silver deposition for signal readout. This method allows sensitive analysis of PEDV and SARS-CoV-2 RNAs in the concentration range from 1 to 1000 nM with the limits of detection as low as 0.47 nM and 0.17 nM, respectively. Good specificities are demonstrated. Thus, the proposed method may have great potential use in the applications of coronaviruses analysis.

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一种简单的电化学生物传感器，用于银沉积的冠状病毒RNA测定。

冠状病毒具有高度传染性，对人类和动物健康构成严重威胁。在这项工作中，开发了一种基于核酸外泌酶III（Exo III）催化消化和银沉积的简单电化学方法用于冠状病毒RNA分析。进行磁分离程序以特异性鉴定靶序列并释放单链DNA修饰的金纳米粒子（AuNPs）。因此，纳米颗粒可以固定在丝网印刷电极上，并催化银沉积用于信号读出。该方法允许对浓度范围为1至1000 nM的PEDV和严重急性呼吸系统综合征冠状病毒2型RNA进行灵敏分析，检测极限分别低至0.47 nM和0.17 nM。证明了良好的特异性。因此，所提出的方法在冠状病毒分析的应用中可能具有巨大的潜在用途。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.