An interventional study of baicalin on neuronal pentraxin-1, neuronal pentraxin-2, and C-reactive protein in Alzheimer's disease rat model.

IF 1.8 4区医学 Q4 NEUROSCIENCES Translational Neuroscience Pub Date : 2023-01-01 DOI:10.1515/tnsci-2022-0298

Jing-Kun Zhao, Si-Jia Hou, Ji-Wei Zhao, Hong-Li Yu, Shu-Rong Duan

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Abstract

Background: Baicalin has been shown to promote spatial learning and neural regeneration, which might increase the differentiation of neural stem cells in Alzheimer's disease (AD) rat models. We aimed to study the role of baicalin on neuronal pentraxin-1 (NPTX-1), neuronal pentraxin-2 (NPTX-2), and C-reactive protein (CRP) in AD model rats.

Methods: The 30 male Sprague Dawley rats were divided into three groups: the control group, the AD model group, and the AD + baicalin group. Then, the Morris water maze was used to verify the effect of baicalin on the memory and spatial learning of rats. Immunohistochemistry and immunofluorescence were used to observe the expression of NPTX-1, NPTX-2, and CRP in brain tissue.

Results: Compared with the AD model group, the AD rats treated with baicalin spent significantly less time finding escape latencies (P = 0.008) and had longer cross-platform times in the target quadrant (P = 0.015). In addition, the AD + baicalin group had significantly higher numbers of hippocampal neurons compared with the AD model group (P < 0.05). Baicalin also obviously decreased the apoptosis of neurons. Moreover, compared with the AD model group, the NPTX-1 and CRP expression in the AD + baicalin group was significantly reduced (P = 0.000) while the expression of NPTX-2 in the brain tissue of AD rats was significantly increased (P = 0.000).

Conclusions: Baicalin can play a therapeutic role by downregulating NPTX-1, upregulating NPTX-2, and downregulating CPR in AD model rats.

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黄芩苷对阿尔茨海默病大鼠模型神经元戊烷素-1、戊烷素-2及c反应蛋白的干预研究。

背景:黄芩苷有促进空间学习和神经再生的作用，这可能会增加阿尔茨海默病(AD)大鼠神经干细胞的分化。本实验旨在研究黄芩苷对AD模型大鼠神经元戊烷素-1 (NPTX-1)、神经元戊烷素-2 (NPTX-2)和c反应蛋白(CRP)的影响。方法:将30只雄性sd大鼠分为对照组、AD模型组和AD +黄芩苷组。然后采用Morris水迷宫验证黄芩苷对大鼠记忆和空间学习的影响。采用免疫组织化学和免疫荧光法观察NPTX-1、NPTX-2、CRP在脑组织中的表达。结果:与AD模型组比较，黄芩苷组AD大鼠寻找逃避潜伏期的时间明显缩短(P = 0.008)，目标象限的跨平台时间明显延长(P = 0.015)。AD +黄芩苷组海马神经元数量显著高于AD模型组(P < 0.05)。黄芩苷还能明显减少神经元的凋亡。与AD模型组比较，AD +黄芩苷组AD大鼠脑组织中NPTX-1和CRP的表达明显降低(P = 0.000)， NPTX-2的表达明显升高(P = 0.000)。结论:黄芩苷可能通过下调NPTX-1、上调NPTX-2、下调CPR对AD模型大鼠有治疗作用。

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来源期刊

Translational Neuroscience NEUROSCIENCES-

CiteScore

3.00

自引率

4.80%

发文量

审稿时长

>12 weeks

期刊介绍： Translational Neuroscience provides a closer interaction between basic and clinical neuroscientists to expand understanding of brain structure, function and disease, and translate this knowledge into clinical applications and novel therapies of nervous system disorders.