An In-depth Guide to the Ultrastructural Expansion Microscopy (U-ExM) of Chlamydomonas reinhardtii.

Nikolai Klena, Giovanni Maltinti, Umut Batman, Gaia Pigino, Paul Guichard, Virginie Hamel
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Abstract

Expansion microscopy is an innovative method that enables super-resolution imaging of biological materials using a simple confocal microscope. The principle of this method relies on the physical isotropic expansion of a biological specimen cross-linked to a swellable polymer, stained with antibodies, and imaged. Since its first development, several improved versions of expansion microscopy and adaptations for different types of samples have been produced. Here, we show the application of ultrastructure expansion microscopy (U-ExM) to investigate the 3D organization of the green algae Chlamydomonas reinhardtii cellular ultrastructure, with a particular emphasis on the different types of sample fixation that can be used, as well as compatible staining procedures including membranes. Graphical overview.

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衣藻超微结构扩展显微镜(U-ExM)深度指南》。
膨胀显微镜是一种创新方法,可使用简单的共聚焦显微镜对生物材料进行超分辨率成像。这种方法的原理依赖于与可膨胀聚合物交联的生物样本的物理各向同性膨胀,并用抗体染色和成像。自首次开发以来,膨胀显微镜已经有了多个改进版本,并适用于不同类型的样本。在这里,我们展示了超微结构膨胀显微镜(U-ExM)在研究绿藻衣藻细胞超微结构的三维组织方面的应用,特别强调了可使用的不同样品固定类型以及包括膜在内的兼容染色程序。图表概览。
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