Determining the Effectiveness of Decontamination with Ionized Hydrogen Peroxide.

IF 0.5 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Applied Biosafety Pub Date : 2020-09-01 DOI:10.1177/1535676020935405

Miguel A Grimaldo, Donald H Bouyer, Claudio L Mafra de Siqueira

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Abstract

Introduction: Ionized Hydrogen Peroxide (iHP) is a new technology used for the decontamination of surfaces or laboratory areas. It utilizes a low concentration of hydrogen peroxide (H₂O₂) mixed with air and ionized through a cold plasma arc. This technology generates reactive oxygen species (ROS) as a means of decontamination.

Objectives: The purpose of this study is to evaluate the diffusion effect of iHP and its decontamination capabilities using biological and enzyme indicators.

Methods: A gas-tight fumigation room with a volume of 880 ft³ was used for the decontamination trials. During the decontamination process, empty animal cages were placed inside to create fumigant distribution restrictions. Spore and enzyme indicators were placed in eleven locations throughout the decontamination room. Generation of iHP was done with the use of TOMI's SteraMist Environmental System and the SteraMist Solution, with 7.8% H₂O₂ at a dose of 0.5 ml per ft³.

Results: For the decontamination of 1hr, 2hrs, 6hrs, and 12hrs, the biological indicators of B. atrophaeus in Stainless Steel (SS) Disk in Tyvek envelope have an inactivation rate of 94%, 97%, 100%, and 100%, respectively. For G. stearothermophilus in SS disk and Tyvek envelope, it has 82%, 68%, 100%, and 100%, respectively and, for G. stearothermophilus in SS strips it has an effective rate of 88%, 67%, 91%, and 100%, respectively.

Conclusion: iHP inactivates spores, and the residual tAK activity indicates a gas-like fumigant diffusion due to the uniformity of the inactivation without the use of oscillating fans as the contact time is extended.

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测定电离过氧化氢去污效果。

简介:离子化过氧化氢(iHP)是一种用于表面或实验室区域净化的新技术。它利用低浓度的过氧化氢(H2O2)与空气混合，并通过冷等离子弧电离。该技术产生活性氧(ROS)作为净化的手段。目的:利用生物和酶指标评价iHP的扩散效果及其去污能力。方法:采用880ft3的气密熏蒸室进行净化试验。在去污过程中，放置了空的动物笼子，以限制熏蒸剂的分发。孢子和酶指示器被放置在整个净化室的11个位置。iHP的生成使用TOMI的SteraMist环境系统和SteraMist溶液，其中含有7.8%的H2O2，剂量为0.5 ml / ft3。结果:对Tyvek包膜不锈钢(SS)盘片进行1h、2h、6h、12h的去污处理，其生物指标的失活率分别为94%、97%、100%、100%。对于SS磁盘和Tyvek包膜中的嗜热G.，其有效率分别为82%、68%、100%和100%;对于SS条中的嗜热G.，其有效率分别为88%、67%、91%和100%。结论:iHP灭活了孢子，残余tAK活性表明，随着接触时间的延长，由于灭活的均匀性，不使用振荡风扇，熏蒸剂的扩散呈气体状。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Applied Biosafety Environmental Science-Management, Monitoring, Policy and Law

CiteScore

2.50

自引率

13.30%

发文量

期刊介绍： Applied Biosafety (APB), sponsored by ABSA International, is a peer-reviewed, scientific journal committed to promoting global biosafety awareness and best practices to prevent occupational exposures and adverse environmental impacts related to biohazardous releases. APB provides a forum for exchanging sound biosafety and biosecurity initiatives by publishing original articles, review articles, letters to the editors, commentaries, and brief reviews. APB informs scientists, safety professionals, policymakers, engineers, architects, and governmental organizations. The journal is committed to publishing on topics significant in well-resourced countries as well as information relevant to underserved regions, engaging and cultivating the development of biosafety professionals globally.