Cerebrospinal fluid proteins in idiopathic intracranial hypertension: An exploratory SWATH proteomics analysis.

IF 2.1 4区生物学 Q3 BIOCHEMICAL RESEARCH METHODS PROTEOMICS – Clinical Applications Pub Date : 2024-01-01 Epub Date: 2023-08-07 DOI:10.1002/prca.202300021

Awadh Kishor Pandit, Shubham Misra, Shantanu Sengupta, Rahul Chakraborty, Praveen Singh, Gyaninder Pal Singh, Swati Phuljhele, Achal K Srivastava, Deepti Vibha, Ajay Garg, Vivek Shankar, Dheeraj Mohania, Garima Shukla, Kameshwar Prasad

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Abstract

Purpose: The pathogenesis of idiopathic intracranial hypertension (IIH) is currently poorly understood. This exploratory study aimed to identify potential cerebrospinal fluid (CSF) biomarkers in IIH cases compared to controls using SWATH-MS proteomics approach.

Experimental design: CSF samples were collected prospectively from IIH cases and control subjects which were subjected to SWATH-MS based untargeted proteomics. Proteins with fold change > 1.5 or < 0.67 and p-value < 0.05 were considered significantly differentially expressed. Data are available via ProteomeXchange with identifier PXD027751. Statistical analysis was conducted in R version 3.6.2.

Results: We included CSF samples from 33 subjects, consisting of 13 IIH cases and 20 controls. A total of 262 proteins were identified in Proteinpilot search. Through SWATH analysis, we quantified 232 proteins. We observed 37 differentially expressed proteins between the two groups with 24 upregulated and 13 downregulated proteins. There were two differential proteins among overweight versus non-overweight IIH cases. Network for 23 proteins was highly connected in the interaction analysis.

Conclusions and clinical relevance: Neurosecretory, neuroendocrine, and inflammatory proteins were predominantly involved in causing IIH. This exploratory study served as a platform to identify 37 differentially expressed proteins in IIH and also showed significant differences between overweight and non-overweight IIH patients.

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特发性颅内高压的脑脊液蛋白质：探索性 SWATH 蛋白质组学分析

目的：特发性颅内高压症（IIH）的发病机制目前尚不清楚。这项探索性研究旨在利用SWATH-MS蛋白质组学方法鉴定特发性颅内高压病例与对照组相比潜在的脑脊液（CSF）生物标志物：实验设计：前瞻性地收集IIH病例和对照组的脑脊液样本，并对其进行基于SWATH-MS的非靶向蛋白质组学分析。结果：33 例 IIH 病例的 CSF 样本中的蛋白质折叠变化大于 1.5：我们纳入了 33 例受试者的 CSF 样本，包括 13 例 IIH 病例和 20 例对照组。通过 Proteinpilot 搜索共鉴定出 262 个蛋白质。通过 SWATH 分析，我们量化了 232 个蛋白质。我们观察到两组之间有 37 个差异表达的蛋白质，其中 24 个上调，13 个下调。超重与非超重 IIH 病例中有两种蛋白质存在差异。在相互作用分析中，23种蛋白质的网络高度关联：神经分泌、神经内分泌和炎症蛋白是导致 IIH 的主要因素。这项探索性研究为确定 IIH 中 37 种差异表达的蛋白质提供了一个平台，同时还显示了超重和非超重 IIH 患者之间的显著差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

PROTEOMICS – Clinical Applications 医学-生化研究方法

CiteScore

5.20

自引率

5.00%

发文量

审稿时长

1 months

期刊介绍： PROTEOMICS - Clinical Applications has developed into a key source of information in the field of applying proteomics to the study of human disease and translation to the clinic. With 12 issues per year, the journal will publish papers in all relevant areas including: -basic proteomic research designed to further understand the molecular mechanisms underlying dysfunction in human disease -the results of proteomic studies dedicated to the discovery and validation of diagnostic and prognostic disease biomarkers -the use of proteomics for the discovery of novel drug targets -the application of proteomics in the drug development pipeline -the use of proteomics as a component of clinical trials.