Regulatory Role of circRNA-0067835 in Behcet Disease through Targeting Micro RNA-155: Implication of ATG1, AKT and MTOR.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2023-04-01 DOI:10.52547/rbmb.12.1.195
Shimaa Saad El-Din, Laila Ahmed Rashed, Doaa Saeed Mohamed, Mervat Eissa, Reham Mohammad Raafat Hamed, Rania Elsayed Hussein
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Abstract

Background: Autophagy has been proven to contribute to maintaining eukaryotic cells' normal intracellular homeostasis, whereas autophagy malfunction may predispose to Behcet Disease (BD). The accumulation of the products of autophagic degradation as well as impairment in autophagic flux in cases with BD, may be attributed to dysregulated miRNA-155 expression. This study attempts to determine the contribution of circRNA-0067835 in miRNA-155-mediated modulation of the autophagy axis as well as to investigate its impact on the production of pro-inflammatory cytokines in BD.

Methods: This study was carried out on 40 cases with BD and 40 healthy control subjects. The collection of serum samples was done before performing a real-time PCR to estimate the relative gene expression of ATG1, AKT, miRNA-155, mTOR, TAB2, and circRNA-0067835. Additionally, IL-1β, IL-17, and TNF-α serum levels were determined by ELISA.

Results: Behcet Disease (BD) patients had significantly upregulated circRNA-0067835, with subsequent downregulation of its target gene, miRNA-155 than controls (P<0.05). In addition, decreased miRNA-155 gene expression was correlated with significantly increased TAB2 gene expression levels in BD patients compared to the controls (P<0.05). Furthermore, enhanced production of pro-inflammatory cytokines was detected in cases with BD than in controls.

Conclusion: The correlation between circRNA-0067835 and miRNA-155 fairly contributes to the regulation of cytokine production in BD via the modulation of autophagy. The investigation of the circRNA-0067835 and the microRNA-155 and their downstream adaptor molecules could be a potential therapeutic agent for BD.

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circRNA-0067835通过靶向微小RNA-155在白塞病中的调节作用:ATG1、AKT和MTOR的意义。
背景:自噬已被证明有助于维持真核细胞正常的细胞内稳态,而自噬功能障碍可能导致白塞病(BD)。BD患者自噬降解产物的积累以及自噬流量的损伤可能归因于miRNA-155表达失调。本研究试图确定circRNA-0067835在miRNA-155介导的自噬轴调节中的作用,并研究其对BD中促炎细胞因子产生的影响。方法:本研究在40例BD患者和40名健康对照受试者中进行。在进行实时PCR以估计ATG1、AKT、miRNA-155、mTOR、TAB2和circRNA-0067835的相对基因表达之前,收集血清样本。此外,通过ELISA测定IL-1β、IL-17和TNF-α的血清水平。结果:白塞病(BD)患者的circRNA-0067835显著上调,随后其靶基因下调,miRNA-155与对照组相比(P结论:circRNA-0067835和miRNA-15之间的相关性通过自噬调节BD中细胞因子的产生。对circRNA-0027835和microRNA-155及其下游衔接分子的研究可能是BD的潜在治疗剂。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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