Testicular cytoprotective effect of glucagon like peptide-1 in diabetic rats involves inhibition of apoptosis, endoplasmic reticulum stress and activation of autophagy.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2023-03-01 DOI:10.4149/gpb_2022064

Elshymaa A Abdel-Hakeem, Nagwa M Zenhom, Sahar A Mokhemer

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引用次数: 1

Abstract

This study aimed to explore the possible cytoprotective effects of exenatide, a glucagon-like peptide-1 (GLP-1) receptor agonist, in the testicles of diabetic rats. Exenatide has numerous advantageous properties in addition to its hypoglycemic effect. However, its impact on testicular tissue in diabetes needs more clarification. Therefore, rats were divided into control, exenatide-treated, diabetic and exenatide-treated diabetic groups. Blood glucose and serum levels of insulin, testosterone, pituitary gonadotropins and kisspeptin-1 were measured. Real-time PCR for beclin-1, p62, mammalian target of rapamycin (mTOR), and AMP-activated protein kinase (AMPK), were estimated in testicular tissue in addition to markers of oxidative stress, inflammation, and endoplasmic reticulum stress. Also, immuno-expression of protein P53, nuclear erythroid factor2 (Nrf2) and vimentin was conducted. Exenatide was able to attenuate diabetic toxic changes and enhance autophagy in testicular tissue. These results indicate the protective effect of exenatide against diabetic testicular dysfunction.

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胰高血糖素样肽-1对糖尿病大鼠睾丸细胞的保护作用包括抑制细胞凋亡、内质网应激和激活自噬。

本研究旨在探讨胰高血糖素样肽-1 (GLP-1)受体激动剂艾塞那肽在糖尿病大鼠睾丸中可能的细胞保护作用。艾塞那肽除了具有降糖作用外，还有许多有利的特性。然而，它对糖尿病患者睾丸组织的影响还需要进一步研究。因此，将大鼠分为对照组、艾塞那肽治疗组、糖尿病组和艾塞那肽治疗组。测定血糖、血清胰岛素、睾酮、垂体促性腺激素和kisspeptin-1水平。在睾丸组织中，除了氧化应激、炎症和内质网应激标志物外，还对哺乳动物雷帕霉素靶蛋白(mTOR) beclin-1、p62、amp活化蛋白激酶(AMPK)进行了实时PCR检测。同时检测P53蛋白、核红细胞因子2 (Nrf2)和vimentin蛋白的免疫表达。艾塞那肽能够减轻糖尿病毒性改变，增强睾丸组织的自噬。提示艾塞那肽对糖尿病睾丸功能障碍具有保护作用。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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