BMP9 maintains the phenotype of HTR-8/Svneo trophoblast cells by activating the SDF1/CXCR4 pathway.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2023-08-07 DOI:10.1186/s12860-023-00487-0
Xue Yang, Lingling Ren, Xiang Chen, Ying Pang, Baoxia Jia, Jing Sun, Xiaofang Quan
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引用次数: 1

Abstract

Background: Bone morphogenetic protein 9 (BMP9) has been shown to regulate processes such as angiogenesis, endothelial dysfunction, and tumorigenesis. However, the role of BMP9 in preeclampsia (PE) is unclear. The purpose of this study was to investigate the role and mechanism of BMP9 in PE.

Methods: The effects of BMP9 on the viability, migration and invasion of HTR-8/Svneo cells were investigated by CCK-8 assay, wound healing assay and Transwell invasion assay. The effect of BMP9 on apoptosis of HTR-8/Svneo cells was detected by flow cytometry. Plasma levels of BMP9, SDF1 and CXCR4 were detected by ELISA kit. qRT-PCR and Western blot were used to detect the expression levels of each gene in the cells.

Results: Overexpression of BMP9 promoted the proliferation and migration of trophoblast cells and inhibited apoptosis. Knockdown of BMP9 had the opposite effect. The levels of BMP9, SDF1 and CXCR4 in the plasma of PE patients were down-regulated, and BMP9 was positively correlated with the levels of SDF1 and CXCR4. BMP9 also significantly upregulated the mRNA and protein levels of SDF1 and CXCR4 in HTR-8/SVneo cells. Further mechanistic studies found that BMP9 promoted the migration and invasion of HTR-8/SVneo cells and inhibited apoptosis by activating the SDF1/CXCR4 pathway.

Conclusion: We demonstrate for the first time that BMP9 promoted the migration and invasion of HTR-8/SVneo cells and inhibits apoptosis by activating the SDF1/CXCR4 pathway. This suggests that BMP9 may be a biomarker molecule for PE.

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BMP9通过激活SDF1/CXCR4通路维持HTR-8/Svneo滋养细胞的表型。
背景:骨形态发生蛋白9 (Bone morphogenetic protein 9, BMP9)已被证明可调节血管生成、内皮功能障碍和肿瘤发生等过程。然而,BMP9在子痫前期(PE)中的作用尚不清楚。本研究的目的是探讨BMP9在PE中的作用和机制。方法:采用CCK-8法、创面愈合法和Transwell侵袭法研究BMP9对HTR-8/Svneo细胞活力、迁移和侵袭的影响。流式细胞术检测BMP9对HTR-8/Svneo细胞凋亡的影响。ELISA试剂盒检测血浆BMP9、SDF1、CXCR4水平。采用qRT-PCR和Western blot检测细胞中各基因的表达水平。结果:BMP9过表达促进滋养细胞增殖和迁移,抑制细胞凋亡。BMP9基因的敲低则有相反的效果。PE患者血浆BMP9、SDF1、CXCR4水平下调,BMP9与SDF1、CXCR4水平呈正相关。BMP9还显著上调HTR-8/SVneo细胞中SDF1和CXCR4的mRNA和蛋白水平。进一步的机制研究发现BMP9通过激活SDF1/CXCR4通路,促进HTR-8/SVneo细胞的迁移和侵袭,抑制细胞凋亡。结论:我们首次证实BMP9通过激活SDF1/CXCR4通路促进HTR-8/SVneo细胞的迁移和侵袭,抑制细胞凋亡。这表明BMP9可能是PE的生物标志物分子。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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