Akram Etemadinia, Amir Seyfoori, Abbas Rahimi Foroushani, Ramin Mazaheri Nezhad Fard, Ronak Bakhtiari
{"title":"Effects of Poly-N-isopropylacrylamide Microgels Containing Antibiofilm Substances on <i>Pseudomonas aeruginosa</i> Isolated from Chronic Wounds.","authors":"Akram Etemadinia, Amir Seyfoori, Abbas Rahimi Foroushani, Ramin Mazaheri Nezhad Fard, Ronak Bakhtiari","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Biofilm formation helps <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) survive in various environments. Microgels can be effective in treatment of bacterial infections. The major aim of this study was to investigate effects of poly-N-isopropylacrylamide microgels (PNIPAM) on <i>P. aeruginosa</i>.</p><p><strong>Methods: </strong>Totally, 100 <i>P. aeruginosa</i> strains were isolated from chronic wound infections. Quantitative assessments of biofilm formation and antibiotic susceptibility were carried out. Furthermore, <i>alg</i>D, <i>las</i>R, and <i>PA</i>2714 genes were amplified to investigate gene frequencies and expression rates.</p><p><strong>Results: </strong>Significant decreases were seen in <i>las</i>R expression in EDTA-treated samples. Significant decreases were observed in expression of <i>alg</i>D and <i>las</i>R treated with xylitol. Decreased expression of <i>PA</i>2714 was seen in samples treated with xylitol with no significance.</p><p><strong>Conclusion: </strong>The PNIPAM containing xylitol or EDTA could penetrate biofilms of <i>P. aeruginosa</i> and significantly decrease expression of <i>las</i>R and <i>alg</i>D. This can be a novel strategy in the management of chronic wounds.</p>","PeriodicalId":8669,"journal":{"name":"Avicenna journal of medical biotechnology","volume":"14 4","pages":"317-320"},"PeriodicalIF":0.0000,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b2/ca/AJMB-14-317.PMC9706250.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Avicenna journal of medical biotechnology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Biofilm formation helps Pseudomonas aeruginosa (P. aeruginosa) survive in various environments. Microgels can be effective in treatment of bacterial infections. The major aim of this study was to investigate effects of poly-N-isopropylacrylamide microgels (PNIPAM) on P. aeruginosa.
Methods: Totally, 100 P. aeruginosa strains were isolated from chronic wound infections. Quantitative assessments of biofilm formation and antibiotic susceptibility were carried out. Furthermore, algD, lasR, and PA2714 genes were amplified to investigate gene frequencies and expression rates.
Results: Significant decreases were seen in lasR expression in EDTA-treated samples. Significant decreases were observed in expression of algD and lasR treated with xylitol. Decreased expression of PA2714 was seen in samples treated with xylitol with no significance.
Conclusion: The PNIPAM containing xylitol or EDTA could penetrate biofilms of P. aeruginosa and significantly decrease expression of lasR and algD. This can be a novel strategy in the management of chronic wounds.
分享
京公网安备 11010802042870号
京ICP备2023020795号-1
求助内容：
应助结果提醒方式：
扫码关注我们
