Extended-volume image-derived models of coronary microcirculation

IF 1.9 4区 医学 Q3 HEMATOLOGY Microcirculation Pub Date : 2023-07-01 DOI:10.1111/micc.12820
Vibujithan Vigneshwaran, Christine Lauren Sy, Bruce H. Smaill, Gregory B. Sands, Nicolas P. Smith
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Abstract

Recent advances in tissue clearing and high‐throughput imaging have enabled the acquisition of extended‐volume microvasculature images at a submicron resolution. The objective of this study was to extract information from this type of images by integrating a sequence of 3D image processing steps on Terabyte scale datasets.

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冠状动脉微循环的大容量图像衍生模型
目的组织清除和高通量成像的最新进展使获得亚微米分辨率的大容量微血管图像成为可能。本研究的目的是通过在tb规模的数据集上集成一系列3D图像处理步骤,从这类图像中提取信息。方法获取3个月大Wistar-Kyoto大鼠心脏全短轴切片的冠状动脉微血管图像。该数据集覆盖13 × 10 × 0.6 mm,分辨率为0.933 × 0.933 × 1.866 μm,占用了700 gb的磁盘空间。我们使用基于块的图像分割,结合高效的图形生成技术,量化了大尺度图像中的微血管。具体来说,我们专注于血管直径达15 μm的微血管系统。结果该管道可在16 h内提取完整的短轴环形态数据。通过分析,我们发现大鼠冠状动脉微血管的微血管长度从6 μm到300 μm不等。然而,它们的分布严重偏向于较短的长度,模态为16.5 μm。相比之下,血管直径范围为3 ~ 15 μm,近似为6.5±2 μm的正态分布。本研究的工具和技术将为其他微循环研究提供服务,本研究的丰富数据将使使用计算机模型分析生物物理机制成为可能。
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来源期刊
Microcirculation
Microcirculation 医学-外周血管病
CiteScore
5.00
自引率
4.20%
发文量
43
审稿时长
6-12 weeks
期刊介绍: The journal features original contributions that are the result of investigations contributing significant new information relating to the vascular and lymphatic microcirculation addressed at the intact animal, organ, cellular, or molecular level. Papers describe applications of the methods of physiology, biophysics, bioengineering, genetics, cell biology, biochemistry, and molecular biology to problems in microcirculation. Microcirculation also publishes state-of-the-art reviews that address frontier areas or new advances in technology in the fields of microcirculatory disease and function. Specific areas of interest include: Angiogenesis, growth and remodeling; Transport and exchange of gasses and solutes; Rheology and biorheology; Endothelial cell biology and metabolism; Interactions between endothelium, smooth muscle, parenchymal cells, leukocytes and platelets; Regulation of vasomotor tone; and Microvascular structures, imaging and morphometry. Papers also describe innovations in experimental techniques and instrumentation for studying all aspects of microcirculatory structure and function.
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