Single laboratory evaluation of umbilical cord blood units processing methodologies for banking.

Laboratory medicine Pub Date : 2024-05-02 DOI:10.1093/labmed/lmad073

Francisco F Dos Santos, Letícia Nunes, Cátia Martins, Margaret Ann Smith, Carla Cardoso

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Abstract

Objective: To compare the efficiency of 3 different processing methods (Sepax, AutoXpress [AXP], and manual processing with hydroxyethyl starch [HES] sedimentation) used at Stemlab during a 10-year period.

Methods: Historical data were compiled and the analytical results obtained for the 3 different methods were compared.

Results: The manual processing (HES) method yielded the highest level of total nucleated cell recovery after processing, and the AXP system yielded the highest CD34+ cell number. The red blood cell reduction was also significantly higher with the HES method. Also, HES showed comparable results to Toticyte technology for umbilical cord blood (UCB) processing.

Conclusion: These results show that the HES method is as effective as automated technologies for UCB volume reduction; hence, it is a suitable methodology for private and public UCB banks. The HES method also proved to be superior to Toticyte technology for medical applications, with higher recovery yields of total nucleated cells after thawing and equivalent CD34+ cell recovery and functionality.

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用于银行的脐带血单位处理方法的单一实验室评估。

目的：比较 Stemlab 在 10 年间使用的 3 种不同处理方法（Sepax、AutoXpress [AXP]、羟乙基淀粉[HES]沉淀法手工处理）的效率：结果：人工处理（HES）法处理后的总核细胞回收率最高，AXP 系统的 CD34+ 细胞数最多。HES 方法的红细胞减少率也明显较高。此外，在处理脐带血（UCB）时，HES 的结果与 Toticyte 技术相当：这些结果表明，在减少脐带血容量方面，HES 方法与自动化技术一样有效；因此，它是一种适用于私营和公共脐带血库的方法。在医疗应用方面，HES 方法也证明优于 Toticyte 技术，解冻后总核细胞的回收率更高，CD34+ 细胞的回收率和功能也相当。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Laboratory medicine

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