Suppressor Mutants: History and Today's Applications.

Q1 Medicine EcoSal Plus Pub Date : 2021-12-15 DOI:10.1128/ecosalplus.ESP-0037-2020
David E Bautista, Joseph F Carr, Angela M Mitchell
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Abstract

For decades, biologist have exploited the near boundless advantages that molecular and genetic tools and analysis provide for our ability to understand biological systems. One of these genetic tools, suppressor analysis, has proven invaluable in furthering our understanding of biological processes and pathways and in discovering unknown interactions between genes and gene products. The power of suppressor analysis lies in its ability to discover genetic interactions in an unbiased manner, often leading to surprising discoveries. With advancements in technology, high-throughput approaches have aided in large-scale identification of suppressors and have helped provide insight into the core functional mechanisms through which suppressors act. In this review, we examine some of the fundamental discoveries that have been made possible through analysis of suppressor mutations. In addition, we cover the different types of suppressor mutants that can be isolated and the biological insights afforded by each type. Moreover, we provide considerations for the design of experiments to isolate suppressor mutants and for strategies to identify intergenic suppressor mutations. Finally, we provide guidance and example protocols for the isolation and mapping of suppressor mutants.

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抑制突变体:历史与当今应用
几十年来,生物学家一直在利用分子和遗传工具及分析为我们了解生物系统提供的近乎无限的优势。抑制因子分析是这些遗传工具中的一种,在进一步了解生物过程和途径以及发现基因和基因产物之间未知的相互作用方面,抑制因子分析已被证明是无价之宝。抑制因子分析的强大之处在于它能够以无偏见的方式发现基因之间的相互作用,往往能带来惊人的发现。随着技术的进步,高通量方法有助于大规模鉴定抑制因子,并有助于深入了解抑制因子发挥作用的核心功能机制。在这篇综述中,我们将探讨通过分析抑制因子突变而获得的一些基本发现。此外,我们还介绍了可以分离出的不同类型的抑制突变体,以及每种类型的抑制突变体所带来的生物学启示。此外,我们还提供了设计实验以分离抑制突变体的注意事项以及鉴定基因间抑制突变的策略。最后,我们还提供了分离和绘制抑制突变体的指导和示例方案。
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来源期刊
EcoSal Plus
EcoSal Plus Immunology and Microbiology-Microbiology
CiteScore
12.20
自引率
0.00%
发文量
4
期刊介绍: EcoSal Plus is the authoritative online review journal that publishes an ever-growing body of expert reviews covering virtually all aspects of E. coli, Salmonella, and other members of the family Enterobacteriaceae and their use as model microbes for biological explorations. This journal is intended primarily for the research community as a comprehensive and continuously updated archive of the entire corpus of knowledge about the enteric bacterial cell. Thoughtful reviews focus on physiology, metabolism, genetics, pathogenesis, ecology, genomics, systems biology, and history E. coli and its relatives. These provide the integrated background needed for most microbiology investigations and are essential reading for research scientists. Articles contain links to E. coli K12 genes on the EcoCyc database site and are available as downloadable PDF files. Images and tables are downloadable to PowerPoint files.
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