Toxicological profile of the Hymenaea courbaril stem bark hydroalcoholic extract using in vitro bioassays and an alternative in vivo Caenorhabditis elegans model.

IF 2.3 4区 医学 Q3 ENVIRONMENTAL SCIENCES Journal of Toxicology and Environmental Health-Part A-Current Issues Pub Date : 2023-09-17 DOI:10.1080/15287394.2023.2237069
Larissa Aline Carneiro Lobo, Peterson Alves Santos, Jayne Torres de Sousa, Jaqueline Nascimento Picada, Sara Elis Bianchi, Valquiria Linck Bassani, Francisco Carlos da Silva, Eduardo Miranda Ethur, Márcia Inês Goettert, Patrícia Pereira
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Abstract

Hymenaea genus has been used in folk medicine in Brazil, but few studies investigated its toxicity profile. Thus, the aim of this study was to determine toxicological parameters of Hymenaea courbaril stem bark hydroalcoholic extract by utilizing three cell lines including murine macrophages (RAW 264.7), mouse fibroblast cells (L929) and human lung fibroblast (MRC-5), as well as Salmonella/microsome assay, and in vivo Caenorhabditis elegans model. The predominant detected phytoconstituents in the extract were coumarins, flavonoids, phenolics, tannins and saponins and by HPLC analysis, astilbin (AST) was found to be the main component. The DPPH assay demonstrated that H. courbaril hydroalcoholic extract exhibited potent antioxidant activity, with an IC50 of 3.12 μg/ml. The extract at concentrations of 400 and 800 μg/ml decreased cell viability 48 hr after treatment in L929 and MRC-5 cell lines. In the Raw 264.7 strain, just the highest concentration (800 μg/ml) lowered cell viability within 48 hr following exposure. The concentration of 100 μg/ml did not markedly affect cell viability in the trypan blue assay. In the alkaline comet assay the extract was found to be non-genotoxic. In the Ames test, the extract exhibited low mutagenic potential without metabolic activation, since only the highest concentrations produced an effect. H. courbaril extract only affected the survival of C. elegans at concentrations of 800 and 1600 μl/ml. These findings demonstrate that H. courbaril extract appears to exert low toxicity as evidenced in vitro and mutagenicity assays; however, the biological relevance of the response of C. elegans survival to safety assessments needs further studies.

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利用体外生物测定法和另一种体内秀丽隐杆线虫模型研究黄膜虫茎皮水醇提取物的毒理学特征。
膜虫属植物在巴西的民间医药中有广泛的应用,但对其毒性研究较少。因此,本研究的目的是利用小鼠巨噬细胞(RAW 264.7)、小鼠成纤维细胞(L929)和人肺成纤维细胞(MRC-5) 3个细胞系,以及沙门氏菌/微粒体实验和秀丽隐杆线虫体内模型,确定膜虫茎皮水醇提取物的毒理学参数。提取液中检测到的主要植物成分为香豆素、黄酮类、酚类、单宁和皂苷,HPLC分析发现主要成分为astilbin (AST)。DPPH实验表明,小檗水醇提取物具有较强的抗氧化活性,IC50为3.12 μg/ml。400和800 μg/ml提取物可降低L929和MRC-5细胞株48小时后的细胞活力。在Raw 264.7菌株中,最高浓度(800 μg/ml)在暴露后48小时内降低了细胞活力。在台盼蓝实验中,100 μg/ml浓度对细胞活力无明显影响。在碱性彗星试验中发现提取物是无基因毒性的。在Ames试验中,提取物表现出低诱变潜力,没有代谢激活,因为只有最高浓度才会产生效果。莪术提取物仅在浓度为800和1600 μl/ml时影响秀丽隐杆线虫的存活。这些发现表明,胡芦巴提取物似乎具有低毒性,正如体外和诱变试验所证明的那样;然而,秀丽隐杆线虫生存反应与安全性评估的生物学相关性需要进一步研究。
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来源期刊
CiteScore
5.20
自引率
19.20%
发文量
46
审稿时长
8-16 weeks
期刊介绍: The Journal of Toxicology and Environmental Health, Part A , Current Issues is an authoritative journal that features strictly refereed original research in the field of environmental sciences, public and occupational health, and toxicology.
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