Electroacupuncture Promotes Motor Function Recovery in MCAO/R Rats by Activating Astrocyte-Related PI3K/AKT Pathway.

IF 0.9 Q4 INTEGRATIVE & COMPLEMENTARY MEDICINE Journal of Acupuncture and Meridian Studies Pub Date : 2022-10-31 DOI:10.51507/j.jams.2022.15.5.322

Xiao-Qing Zhang, Yi-He Wang, Li Sun, Bao-Qiang Dong, Yue-Jiao Sui, Jia-Zi Dong, Yang Han

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引用次数: 1

Abstract

Background: Electroacupuncture (EA) is a widely used traditional Chinese medicine method to manage various diseases, including cerebral ischemia-reperfusion injury (CIRI).

Objectives: We assessed the neuroprotective effects of EA and examined its mechanism in a rat model of the middle cerebral artery occlusion-reperfusion (MCAO/R). The gait analysis was performed to evaluate the neuroprotective effects. Western blot and immunohistochemistry assays were carried out to determine the molecular mechanisms of EA.

Methods: Male SD rats were randomly divided into the sham operation group, right MCAO/R group, and EA group. EA was administered every day (4/20 Hz, 10 min/1 d) at the following acupoints: Baihui (DU20), Yintang (EX-HN3), and Zusanli (ST36). Gait and motor function were analyzed from day 8 onward.

Results: The plantar support and balance coordination of MCAO/R rats decreased, and the cellular structure of the ischemic penumbra was unclear. EA improved the gait dynamics of the rats, adjusted the cell structure, further activated astrocytes, and increased the expression and phosphorylation of phosphoinositide 3-kinase/protein kinase B (PI3K/PKB or AKT).

Conclusion: EA promoted astrocyte-related effects in the rat model. Our findings suggest that the neuroprotective mechanism of EA may be related to the activation of the PI3K/AKT signaling pathway. The intervention enhanced brain protection and improved motor functions.

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电针通过激活星形胶质细胞相关PI3K/AKT通路促进MCAO/R大鼠运动功能恢复。

背景:电针(EA)是一种广泛应用于治疗多种疾病的中医方法，包括脑缺血再灌注损伤(CIRI)。目的:在大鼠大脑中动脉闭塞-再灌注(MCAO/R)模型中评估EA的神经保护作用并探讨其机制。步态分析评估神经保护作用。方法:雄性SD大鼠随机分为假手术组、右MCAO/R组和EA组。每日(4/20 Hz, 10 min/1 d)给药:百会(DU20)、音堂(EX-HN3)、足三里(ST36)。从第8天开始分析步态和运动功能。结果:MCAO/R大鼠足底支持和平衡协调能力下降，缺血半暗带细胞结构不清。EA改善了大鼠的步态动力学，调节了细胞结构，进一步激活了星形胶质细胞，增加了磷酸肌肽3-激酶/蛋白激酶B (PI3K/PKB或AKT)的表达和磷酸化。结论:EA促进了大鼠模型中星形胶质细胞的相关作用。我们的研究结果表明，EA的神经保护机制可能与激活PI3K/AKT信号通路有关。干预增强了大脑保护，改善了运动功能。

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来源期刊

Journal of Acupuncture and Meridian Studies INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

1.50

自引率

10.00%

发文量

审稿时长

12 weeks

期刊介绍： The Journal of Acupuncture and Meridian Studies is a quarterly, peer-reviewed journal featuring high-quality studies related to basic and clinical acupuncture and meridian research. It also includes new paradigm of integrative research, covering East–West and traditional–modern medicine. Manuscripts should fall into one of the categories: topical review, original research paper, brief ... click here for full Aims & Scope The Journal of Acupuncture and Meridian Studies is a quarterly, peer-reviewed journal featuring high-quality studies related to basic and clinical acupuncture and meridian research. It also includes new paradigm of integrative research, covering East–West and traditional–modern medicine.