High sensitivity CIP2A detection for oral cancer using a rapid transistor-based biosensor module.

IF 1.5 4区工程技术 Q3 ENGINEERING, ELECTRICAL & ELECTRONIC Journal of Vacuum Science and Technology B:Nanotechnology and Microelectronics Pub Date : 2023-01-01 Epub Date: 2022-12-13 DOI:10.1116/6.0002175

Minghan Xian, Jenna L Stephany, Chan-Wen Chiu, Chao-Ching Chiang, Fan Ren, Cheng-Tse Tsai, Siang-Sin Shan, Yu-Te Liao, Josephine F Esquivel-Upshaw, Stephen J Pearton

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引用次数: 1

Abstract

Oral squamous cell carcinoma (OSCC) is one of the most common lip and oral cavity cancer types. It requires early detection via various medical technologies to improve the survival rate. While most detection techniques for OSCC require testing in a centralized lab to confirm cancer type, a point of care detection technique is preferred for on-site use and quick result readout. The modular biological sensor utilizing transistor-based technology has been leveraged for testing CIP2A, and optimal transistor gate voltage and load resistance for sensing setup was investigated. Sensitivities of 1 × 10^-15 g/ml have been obtained for both detections of pure CIP2A protein and HeLa cell lysate using identical test conditions via serial dilution. The superior time-saving and high accuracy testing provides opportunities for rapid clinical diagnosis in the medical space.

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基于快速晶体管的生物传感器模块的口腔癌高灵敏度CIP2A检测。

口腔鳞状细胞癌(OSCC)是唇和口腔最常见的癌症类型之一。它需要通过各种医疗技术进行早期检测，以提高生存率。虽然大多数OSCC检测技术需要在集中实验室进行检测以确认癌症类型，但对于现场使用和快速读出结果而言，护理点检测技术是首选。利用基于晶体管技术的模块化生物传感器对CIP2A进行了测试，并对传感器设置的最佳晶体管栅极电压和负载电阻进行了研究。在相同的测试条件下，通过连续稀释，对纯CIP2A蛋白和HeLa细胞裂解液的检测灵敏度均为1 × 10-15 g/ml。卓越的省时和高精度的测试为医疗领域的快速临床诊断提供了机会。

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