Alpha, Beta, and Gamma Human Papillomaviruses in Genital Lichen Sclerosus: A Retrospective Cross-Sectional Study.

IF 2.4 4区医学 Q2 OBSTETRICS & GYNECOLOGY Journal of Lower Genital Tract Disease Pub Date : 2023-07-01 Epub Date: 2023-04-11 DOI:10.1097/LGT.0000000000000741

Eugenia Giuliani, Francesca Rollo, Carlo Cota, Tarik Gheit, Luisa Galati, Sandrine McKay-Chopin, Marinella Tedesco, Emilia Migliano, Maria Benevolo, Aldo Morrone, Maria Gabriella Donà, Alessandra Latini

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Abstract

Background: Lichen sclerosus (LS) is an inflammatory disease mostly arising at the genital level. It is unclear whether human papillomaviruses (HPVs) have an etiological significance in LS, and data on their prevalence in patients with LS are controversial.

Objectives: The authors assessed alpha, beta, and gamma HPV prevalence in patients with genital LS. The association of HPV positivity with demographic and clinical factors was also investigated.

Methods: One hundred thirty-two formalin-fixed, paraffin-embedded LS samples (2016-2020) were retrieved from the archives of a pathology department. Alpha HPVs were genotyped with the INNO-LiPA HPV Genotyping Extra II kit. Beta and gamma HPVs were searched by multiplex Polymerase Chain Reaction. Immunostaining for p16 INK4a was performed on high-risk HPV-positive samples.

Results: Patients had a median age of 61 years, were mostly women ( n = 73, 55.3%), and with an early disease stage ( n = 79, 59.8%). Alpha HPVs were detected in 12/132 cases (9.1%). Among the 5 high-risk HPV-positive cases, only 2 displayed a strong and diffuse p16 INK4a staining. Beta genus was the most prevalent (35/132, 26.5%) and HPV5 was the most frequent beta genotype (25/132, 18.9%). There were 3 gamma HPV-positive cases among those with a valid result (3/131, 2.3%). Multiple infections with genotypes belonging to different genera were infrequent (3/131, 2.3%). No significant differences in the prevalence of the individual genera were observed according to sex and disease stage.

Conclusions: Of the 3 HPV genera, beta genus showed the highest prevalence. Further research is needed to clarify whether the presence of beta HPVs in genital LS has a clinical significance.

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生殖器硬皮病中的α、β和γ人类乳头瘤病毒：回顾性横断面研究。

背景：硬皮病（LS）是一种炎症性疾病，主要发生在生殖器部位。目前尚不清楚人类乳头瘤病毒（HPV）是否是 LS 的致病因素，有关其在 LS 患者中流行情况的数据也存在争议：作者评估了生殖器 LS 患者中的α、β和γ HPV 感染率。目的：作者评估了生殖器 LS 患者中α、β和γ HPV 的感染率，并研究了 HPV 阳性与人口统计学和临床因素的关系：方法：从病理科档案中检索了 132 份福尔马林固定、石蜡包埋的 LS 样本（2016-2020 年）。使用 INNO-LiPA HPV Genotyping Extra II 试剂盒对α HPV 进行基因分型。β和γ型HPV通过多重聚合酶链反应进行搜索。对高危 HPV 阳性样本进行 p16 INK4a 免疫染色：患者的中位年龄为 61 岁，大多数为女性（73 人，占 55.3%），处于疾病早期（79 人，占 59.8%）。12/132例（9.1%）中检测到α HPV。在 5 个高危 HPV 阳性病例中，只有 2 个病例的 p16 INK4a 染色呈弥漫性强阳性。贝塔型是最常见的基因型（35/132，26.5%），HPV5 是最常见的贝塔型基因型（25/132，18.9%）。在有效结果中，有 3 例γ HPV 阳性病例（3/131，2.3%）。不同属的基因型多重感染的情况并不多见（3/131，2.3%）。根据性别和疾病阶段的不同，各属的感染率也无明显差异：结论：在 3 个 HPV 属中，β 属的流行率最高。结论：在 3 个 HPV 属中，贝塔属的发病率最高，需要进一步研究以明确生殖器 LS 中贝塔属 HPV 的存在是否具有临床意义。

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来源期刊

Journal of Lower Genital Tract Disease OBSTETRICS & GYNECOLOGY-

CiteScore

6.80

自引率

8.10%

发文量

158

审稿时长

6-12 weeks

期刊介绍： The Journal of Lower Genital Tract Disease is the source for the latest science about benign and malignant conditions of the cervix, vagina, vulva, and anus. The Journal publishes peer-reviewed original research original research that addresses prevalence, causes, mechanisms, diagnosis, course, treatment, and prevention of lower genital tract disease. We publish clinical guidelines, position papers, cost-effectiveness analyses, narrative reviews, and systematic reviews, including meta-analyses. We also publish papers about research and reporting methods, opinions about controversial medical issues. Of particular note, we encourage material in any of the above mentioned categories that is related to improving patient care, avoiding medical errors, and comparative effectiveness research. We encourage publication of evidence-based guidelines, diagnostic and therapeutic algorithms, and decision aids. Original research and reviews may be sub-classified according to topic: cervix and HPV, vulva and vagina, perianal and anal, basic science, and education and learning. The scope and readership of the journal extend to several disciplines: gynecology, internal medicine, family practice, dermatology, physical therapy, pathology, sociology, psychology, anthropology, sex therapy, and pharmacology. The Journal of Lower Genital Tract Disease highlights needs for future research, and enhances health care. The Journal of Lower Genital Tract Disease is the official journal of the American Society for Colposcopy and Cervical Pathology, the International Society for the Study of Vulvovaginal Disease, and the International Federation of Cervical Pathology and Colposcopy, and sponsored by the Australian Society for Colposcopy and Cervical Pathology and the Society of Canadian Colposcopists.