An Assessment of the Serum Activity of ADH and ALDH in Patients with Primary Biliary Cholangitis

IF 2.9 4区 医学 Q3 IMMUNOLOGY Archivum Immunologiae et Therapiae Experimentalis Pub Date : 2022-12-28 DOI:10.1007/s00005-022-00667-4
Blanka Wolszczak-Biedrzycka, Anna Bieńkowska, Elżbieta Zasimowicz, Grzegorz Biedrzycki, Justyna Dorf, Wojciech Jelski
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引用次数: 1

Abstract

Primary biliary cholangitis (PBC; previously known as primary biliary cirrhosis) is a chronic inflammation-induced cholestatic process in the liver. Antimitochondrial antibodies (AMAs) are observed in around 90% of patients, which suggests that PBC is an autoimmune disease. Alcohol dehydrogenase (ADH), ADH isoenzymes and aldehyde dehydrogenase (ALDH) are localized in the liver, and they are useful markers of liver dysfunction. In this study, the activity of total ADH, ADH isoenzymes and ALDH was evaluated in the blood serum of patients with PBC. The experimental group comprised 50 PBC patients, both male and female, aged 28–67. The control group consisted of 50 healthy subjects, both male and female, aged 25–65. The serum activity of class I ADH, class II ADH and ALDH was measured by spectrofluorophotometry, whereas total ADH and class III ADH activity was determined by photometry methods. The activity of class I ADH and total ADH was significantly higher in the experimental group than in the control group (p < 0.001). An increase in class I ADH and total ADH activity indicates that the isoenzyme class I ADH is released by compromised liver cells and can be useful diagnostic markers of PBC.

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原发性胆道性胆管炎患者血清ADH和ALDH活性的评价
原发性胆管炎;原发性胆汁性肝硬化(以前称为原发性胆汁性肝硬化)是肝脏慢性炎症诱导的胆汁淤积过程。约90%的患者存在抗线粒体抗体(AMAs),提示PBC是一种自身免疫性疾病。乙醇脱氢酶(ADH), ADH同工酶和醛脱氢酶(ALDH)定位于肝脏,它们是肝脏功能障碍的有用标志物。本研究测定了PBC患者血清总ADH、ADH同工酶和ALDH的活性。实验组50例PBC患者,男女均有,年龄28-67岁。对照组由50名年龄在25-65岁的健康男性和女性组成。血清ⅰ类、ⅱ类ADH和ALDH活性采用荧光光度法测定,总ADH和ⅲ类ADH活性采用光度法测定。试验组ⅰ类ADH活性和总ADH活性显著高于对照组(p < 0.001)。I类ADH和总ADH活性的增加表明I类ADH同工酶由受损的肝细胞释放,可以作为PBC的有用诊断标志物。
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来源期刊
CiteScore
5.90
自引率
0.00%
发文量
26
审稿时长
>12 weeks
期刊介绍: Archivum Immunologiae et Therapiae Experimentalis (AITE), founded in 1953 by Ludwik Hirszfeld, is a bimonthly, multidisciplinary journal. It publishes reviews and full original papers dealing with immunology, experimental therapy, immunogenetics, transplantation, microbiology, immunochemistry and ethics in science.
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