Microscopic and Molecular Investigation of Intestinal Microsporidia in HIV + /AIDS and Cancer Patients Undergoing Chemotherapy in Mazandaran Province, North of Iran

IF 1.2 3区农林科学 Q4 PARASITOLOGY Acta Parasitologica Pub Date : 2023-08-11 DOI:10.1007/s11686-023-00704-y

Bentolhoda Ahmadi, Shahabeddin Sarvi, Shahrbanoo Keihanian, Lotfollah Davoudi, Ahmad Daryani, Hamed Mirjalali, Ehsan Ahmadpour, Mitra Sadeghi, Seyed Ali Shariatzadeh, Saeid Shakeri, Seyed Abdollah Hosseini

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Abstract

Background

The aim of this study was to identify Enterocytozoon bieneusi and Encephalitozoon spp. in fecal samples of HIV + /AIDS and cancer patients undergoing chemotherapy, and comparing the results to healthy individuals in Mazandaran province, north of Iran.

Methods

Stool samples were collected from 50 HIV + /AIDS patients, 50 cancer patients, and 50 healthy samples referred to medical centers in north of Iran. Stool samples were kept in 2.5% potassium dichromate at 4 °C, and stained by modified trichrome for light microscopy examination. The multiplex/nested-PCR targeted the small subunit ribosomal RNA (SSU rRNA) gene. To characterize genotypes, the nested PCR products sequenced by Bioneer Company and was subjected to phylogenetic analyses.

Results

Ten of 50 samples (20%) of HIV + /AIDS patients, 5 of 50 samples (10%) of cancer patients, and 1 of healthy individuals (2%) were microscopically positive. From 50 HIV + / AIDS patients, E. bieneusi and Encephalitozoon spp. were detected in 10 (20%) and 6 (12%) cases, respectively. Furthermore, among cancer patients, 7 (14%) and 2 (4%) cases were E. bieneusi and Encephalitozoon spp., respectively. Out of 50 samples of healthy individuals, only 3 (6%) cases of E. bieneusi were observed. The genotypes D and M were detected among positive samples of E. bieneusi.

Conclusions

E. bieneusi and then Encephalitozoon spp. are common intestinal microsporidia in HIV + /AIDS patients and cancer patients undergoing chemotherapy in Mazandaran province. E. bieneusi genotype D seems to be the predominant genotype in Mazandaran province. Due to the considerable prevalence of intestinal microsporidia, physicians are advised to pay more attention to this opportunistic infection in high-risk groups.

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伊朗北部马赞达兰省接受化疗的HIV + /艾滋病和癌症患者肠道微孢子虫的显微和分子研究

本研究的目的是在伊朗北部马赞达兰省接受化疗的HIV + /艾滋病和癌症患者的粪便样本中鉴定出双胞虫和脑囊虫，并将结果与健康个体进行比较。方法收集伊朗北部地区50例HIV + /AIDS患者、50例癌症患者和50例健康患者的粪便样本。粪便标本在2.5%重铬酸钾中保存4℃，经改良三色染色，光镜检查。多重/巢式pcr针对小亚基核糖体RNA (SSU rRNA)基因。为了确定基因型，巢式PCR产物由Bioneer公司测序，并进行系统发育分析。结果50例HIV + /AIDS患者(占20%)、50例癌症患者(占10%)、健康人群(占2%)镜检阳性。在50例HIV + / AIDS患者中，分别检出10例(20%)和6例(12%)布氏埃希菌和脑囊虫。此外，在癌症患者中，分别有7例(14%)和2例(4%)为双胞杆菌和脑囊虫。在50个健康个体样本中，仅观察到3例(6%)比氏伊夫氏杆菌病例。阳性标本中检出D、M基因型。bieneusi和Encephalitozoon spsp是Mazandaran省HIV + /AIDS患者和癌症化疗患者中常见的肠道微孢子虫。基因型D似乎是马赞达兰省的主要基因型。由于肠道微孢子虫相当普遍，建议医生在高危人群中更加注意这种机会性感染。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.