Quantification of Botrytis cinerea Growth in Arabidopsis thaliana.

Patricia Scholz, Kent D Chapman, Till Ischebeck, Athanas Guzha
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Abstract

Yield losses attributed to plant pathogens pose a serious threat to plant productivity and food security. Botrytis cinerea is one of the most devastating plant pathogens, infecting a wide array of plant species; it has also been established as a model organism to study plant-pathogen interactions. In this context, development of different assays to follow the relative success of B. cinerea infections is required. Here, we describe two methods to quantify B. cinerea development in Arabidopsis thaliana genotypes through measurements of lesion development and quantification of fungal genomic DNA in infected tissues. This provides two independent techniques that are useful in assessing the susceptibility or tolerance of different Arabidopsis genotypes to B. cinerea. Key features Protocol for the propagation of the necrotrophic plant pathogen fungus Botrytis cinerea and spore production. Two methods of Arabidopsis thaliana infection with the pathogen using droplet and spray inoculation. Two readouts, either by measuring lesion size or by the quantification of fungal DNA using quantitative PCR. The two methods are applicable across plant species susceptible the B. cinerea. Graphical overview A simplified overview of the droplet and spray infection methods used for the determination of B. cinerea growth in different Arabidopsis genotypes.

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拟南芥灰霉病菌生长的定量研究。
植物病原体造成的产量损失对植物生产力和粮食安全构成严重威胁。灰霉病菌是最具破坏性的植物病原体之一,感染广泛的植物物种;它也被确立为研究植物与病原体相互作用的模式生物。在这种情况下,需要开发不同的检测方法来跟踪灰绿杆菌感染的相对成功。在这里,我们描述了两种方法,通过测量病变发展和真菌基因组DNA在感染组织中量化拟南芥基因型中灰绿杆菌的发展。这为评估不同拟南芥基因型对灰孢杆菌的易感性或耐受性提供了两种独立的技术。主要特点:坏死性植物病原菌灰霉病菌的繁殖和孢子的产生。拟南芥侵染病原菌的两种方法分别为液滴接种和喷雾接种。两个读数,要么通过测量病变大小或通过真菌DNA定量PCR定量。两种方法均适用于对灰霉病敏感的植物。简要介绍了测定不同基因型拟南芥中灰绿芽孢杆菌生长的液滴和喷雾感染方法。
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