Growth, tolerance, and enzyme activities of Trichoderma strains in culture media added with a pyrethroids-based insecticide

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-01-01 DOI:10.1016/j.ram.2023.06.004

Caliope Mendarte-Alquisira, Alejandro Alarcón, Ronald Ferrera-Cerrato

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Abstract

The application of pyrethroids and carbamates represents an environmental risk and may exert adverse effects on beneficial microorganisms such as Trichoderma, which contribute to the biocontrol of several fungal phytopathogens. This research evaluated the tolerance of several strains of Trichoderma to a selected culture medium contaminated with a commercial insecticide (H24®) composed of pyrethroids, permethrin and prallethrin, and carbamate propoxur, and determined the influence of this insecticide on the release of enzymes such as chitinases, peroxidases, and endoglucanases by a consortium of selected Trichoderma strains grown in liquid culture medium. Four out of 10 Trichoderma strains showed tolerance to 200 ppm (∼48.3% of growth) of the commercial insecticide after 96 h of exposure to a contaminated solid medium. After eight days of growth in liquid culture, the insecticide enhanced extracellular protein content and peroxidase activities in the Trichoderma consortium but decreased both chitinase and glucanase activities. These fungal responses should be considered when implementing strategies that combine alternative pesticides and fungal biocontrollers for managing fungal phytopathogens.

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加入拟除虫菊酯类杀虫剂的培养基中毛霉菌株的生长、耐受性和酶活性

使用拟除虫菊酯和氨基甲酸酯会对环境造成危害，并可能对毛霉等有益微生物产生不利影响。这项研究评估了几种毛霉菌株对由拟除虫菊酯、氯菊酯、炔菊酯和氨基甲酸酯类丙硫磷组成的商业杀虫剂（H24®）污染的特定培养基的耐受性，并确定了这种杀虫剂对在液体培养基中生长的特定毛霉菌株释放几丁质酶、过氧化物酶和内切葡聚糖酶等酶的影响。10 株毛霉中有 4 株在接触受污染的固体培养基 96 小时后，显示出对 200 ppm（生长量的 48.3%）商用杀虫剂的耐受性。在液体培养基中生长八天后，杀虫剂提高了毛霉菌群的胞外蛋白质含量和过氧化物酶活性，但降低了几丁质酶和葡聚糖酶活性。在实施结合替代杀虫剂和真菌生物控制剂来管理真菌植物病原体的策略时，应考虑到这些真菌反应。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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