Increased Apoptosis in Subcortical Regions of The Visual Pathway in Offspring Born to Diabetic Rats.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2023-08-01 DOI:10.22074/cellj.2023.1989649.1232

Nasim Alipour, Somaye Fallahnezhad, Javad Bagheri, Hamideh Babaloo, Fatemeh Tahmasebi, Ghasem Sazegar, Hossein Haghir

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引用次数: 1

Abstract

Objective: Diabetes in pregnancy is a prevalent disease that can affect the central nervous system of the fetus by hyperglycemia. This study aimed to investigate the impact of maternal diabetes on neuronal apoptosis in the superior colliculus (SC) and the lateral geniculate nucleus (LGN) in male neonates born to diabetic mothers.

Materials and methods: In this experimental study, female adult rats were separated into three groups: control, diabetic (induced using an intraperitoneal injection of streptozotocin), and insulin-treated diabetic [diabetes controlled by subcutaneous neutral protamine hagedorn (NPH)-insulin injection]. Male neonates from each group were euthanized on 0, 7, and 14 postnatal days (P0, P7, and P14, respectively), and apoptotic cells were identified using TUNEL staining.

Results: The numerical density per unit area (NA) of apoptotic cells was significantly higher in SC and the dorsal LGN (dLGN) in neonates born to the diabetic rats compared to the control group at P0, P7, and P14. However, insulin treatment normalized the number of apoptotic cells.

Conclusion: This study demonstrated that maternal diabetes increased apoptosis in dLGN and SC of male neonates at P0, P7, and P14.

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糖尿病大鼠后代视觉通路皮质下区域细胞凋亡增加。

目的：妊娠期糖尿病是一种常见疾病，可通过高血糖影响胎儿的中枢神经系统。本研究旨在研究母亲糖尿病对糖尿病母亲所生男性新生儿上丘（SC）和外侧膝状体核（LGN）神经元凋亡的影响。材料和方法：在本实验研究中，成年雌性大鼠分为三组：对照组、糖尿病组（腹腔注射链脲佐菌素诱导）和胰岛素治疗的糖尿病组（皮下注射中性鱼精蛋白-胰岛素控制糖尿病）。每组雄性新生儿在出生后0、7和14天（分别为P0、P7和P14）实施安乐死，并使用TUNEL染色鉴定凋亡细胞。结果：与对照组相比，在P0、P7和P14，糖尿病大鼠所生新生儿的SC和背侧LGN中凋亡细胞的单位面积数值密度（NA）显著更高。然而，胰岛素治疗使凋亡细胞的数量正常化。结论：本研究表明，母体糖尿病在P0、P7和P14时增加了男性新生儿dLGN和SC的细胞凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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